Abstract
The spread of antibiotic resistance is rapidly threatening the effectiveness of antibiotics in the clinical setting. Many infections are being caused by known and unknown pathogenic bacteria that are resistant to many or all antibiotics currently available. Empedobacter falsenii is a nosocomial pathogen that can cause human infections. E. falsenii Wf282 strain was found to be resistant to many antibiotics, including carbapenems and colistin. Whole-genome shotgun sequencing of the strain was performed, and distinct features were identified. A novel metallo-β-lactamase, named EBR-2, was found, suggesting a potential role of E. falsenii as a reservoir of β-lactamases and other resistance determinants also found in its genome. The EBR-2 protein showed the highest catalytic efficiency for penicillin G as compared to meropenem and ampicillin and was unable to hydrolyze cefepime. The results described in this work broaden the current understanding of the role of β-lactamases in the Flavobacteriaceae family and suggest that E. falsenii Wf282 may be a reservoir of these novel resistance determinants.
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Acknowledgements
Secretaría de Ciencia y Técnica de la Universidad de Buenos Aires” (UBACyT) to CV, Buenos Aires, Argentina. GMT, SM, and KC were supported by a Post-doctoral fellowship and doctoral fellowship from CONICET, and grant MHIRT 2T37MD001368 from the National Institute on Minority Health and Health Disparities, National Institute of Health, respectively. AI is a member of the “Sistema Nacional de Investigadores” and “PEDECIBA,” Uruguay.
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Supplementary material 1 (PDF 77 KB) Maximum likelihood phylogenetic tree of the Flavobacteriaceae family based on 21 conserved ribosomal protein-coding genes. A blue arrow indicates the phylogenetic position of E. falsenii Wf282. Blue branches define the monophyletic group of the closely related genomes of E. falsenii Wf282, bootstrap support is indicated next to nodes. Assembly code of each genome is also indicated. The phylogeny is arbitrary rooted using the midpoint rooting method.
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Collins, C., Almuzara, M., Saigo, M. et al. Whole-Genome Analysis of an Extensively Drug-Resistance Empedobacter falsenii Strain Reveals Distinct Features and the Presence of a Novel Metallo-ß-Lactamase (EBR-2). Curr Microbiol 75, 1084–1089 (2018). https://doi.org/10.1007/s00284-018-1498-9
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DOI: https://doi.org/10.1007/s00284-018-1498-9