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Complete genome sequencing and comparative analysis of the linezolid-resistant Enterococcus faecalis strain DENG1

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Abstract

Genome level analysis of bacterial strains provides information on genetic composition and resistance mechanisms to clinically relevant antibiotics. To date, whole genome characterization of linezolid-resistant Enterococcus faecalis isolated in the clinic is lacking. In this study, we report the entire genome sequence, genomic characteristics and virulence factors of a pathogenic E. faecalis strain, DENG1. Our results showed considerable differences in genomic characteristics and virulence factors compared with other E. faecalis strains (V583 and OG1RF). The genome of this LZD-resistant E. faecalis strain can be used as a reference to study the mechanism of LZD resistance and the phylogenetic relationship of E. faecalis strains worldwide.

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Abbreviations

LZD:

Linezolid

MRSA:

Methicillin-resistant Staphylococcus aureus

VRE:

Vancomycin-resistant Enterococci

PAIs:

Pathogenicity islands

References

  • Ager S, Gould K (2012) Clinical update on linezolid in the treatment of gram-positive bacterial infections. Infect Drug Resist 5:87–102

    CAS  PubMed Central  PubMed  Google Scholar 

  • Arias CA, Murray BE (2012) The rise of the Enterococcus: beyond vancomycin resistance. Nat Rev Microbiol 10:266–278

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA et al (2008) The RAST server: rapid annotations using subsystems technology. BMC Genomics 9:75

    Article  PubMed Central  PubMed  Google Scholar 

  • Billal DS, Feng J, Leprohon P, Légaré D, Ouellette M (2011) Whole genome analysis of linezolid resistance in Streptococcus pneumoniae reveals resistance and compensatory mutations. BMC Genomics 12:512

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Bose M, Barber RD (2006) Prophage finder: a prophage loci prediction tool for prokaryotic genome sequences. In Silico Biol 6:223–227

    CAS  PubMed  Google Scholar 

  • Brede DA, Snipen LG, Ussery DW, Nederbragt AJ, Nes IF (2011) Complete genome sequence of the commensal Enterococcus faecalis 62, isolated from a healthy Norwegian infant. J Bacteriol 193:2377–2378

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Darling AE, Mau B, Perna NT (2010) Progressive mauve: multiple genome alignment with gene gain, loss and rearrangement. PLoS ONE 5:e11147

    Article  PubMed Central  PubMed  Google Scholar 

  • Feng J, Lupien A, Gingras H, Wasserscheid J, Dewar K, Légaré D et al (2009) Genome sequencing of linezolid-resistant Streptococcus pneumoniae mutants reveals novel mechanisms of resistance. Genome Res 19:1214–1223

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Long KS, Vester B (2012) Resistance to linezolid caused by modifications at its binding site on the ribosome. Antimicrob Agents Chemother 56:603–612

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • McBride SM, Coburn PS, Baghdayan AS, Willems RJ, Grande MJ, Shankar N et al (2009) Genetic variation and evolution of the pathogenicity island of Enterococcus faecalis. J Bacteriol 191:3392–3402

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Paulsen IT, Banerjei L, Myers GS et al (2003) Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science 299:2071–2074

    Article  CAS  PubMed  Google Scholar 

  • Zischka M, Kuenne C, Blom J, Dabrowski PW, Linke B, Hain T et al (2012) Complete genome sequence of the porcine isolate Enterococcus faecalis D32. J Bacteriol 194:5490–5491

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by Grants from the Key Subject Fund of Shenzhen Government and Shenzhen Council for Technology and Creation (No.: JCYJ20130402151227180), Shenzhen Scientific Research Program of the People’s Republic of China (No.: 201303179). We thank Zhang Yitong for technical assistance. We thank the Shanghai Majorbio Bio-pharm Technology Corporation for allowing this work to progress in their laboratory and providing samples.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Shenzhen Key Lab for Endogenous Infection, Department of Infectious Diseases, The Affiliated Shenzhen Nanshan Hospital, Guangdong Medical College, No 89, Taoyuan Road, Nanshan district, Shenzhen, 518052, China

    Zhijian Yu, Zhong Chen, Hang Cheng, Jinxin Zheng, Duoyun Li, Xiangbin Deng, Weiguang Pan, Weizhi Yang & Qiwen Deng

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  1. Zhijian Yu
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  2. Zhong Chen
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  3. Hang Cheng
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  4. Jinxin Zheng
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  5. Duoyun Li
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  6. Xiangbin Deng
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  7. Weiguang Pan
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  8. Weizhi Yang
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  9. Qiwen Deng
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Corresponding author

Correspondence to Qiwen Deng.

Additional information

Communicated by Erko Stackebrandt.

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Yu, Z., Chen, Z., Cheng, H. et al. Complete genome sequencing and comparative analysis of the linezolid-resistant Enterococcus faecalis strain DENG1. Arch Microbiol 196, 513–516 (2014). https://doi.org/10.1007/s00203-014-0986-y

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  • DOI: https://doi.org/10.1007/s00203-014-0986-y

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