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Aquichromatium aeriopus gen. nov., sp. nov., A Non-phototrophic Aerobic Chemoheterotrophic Bacterium, and Proposal of Aquichromatiaceae fam. nov. in the Order Chromatiales

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Abstract

A gram-staining negative, non-motile, aerobic chemoheterotrophic, ovoid or short rod-shaped bacterium, designated as J89T, was isolated from a seawater sample collected from the coast of Yellow Sea in Qingdao, China. The strain grew at salinities of 1.0–6.0% (w/v) NaCl (optimum, 3.0%). Growth occurred at pH 6.0–9.0 (optimum, pH 7.0) and at 10–35 °C (optimum, 25–30 °C). The genomic DNA G+C content was determined to be 59.3 mol%. Q-8 was detected as the respiratory quinone. The major fatty acids (>10%) were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), and C16:0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified phospholipids, and an unidentified polar lipid. Comparison of the 16S rRNA gene sequence indicated that the strain was most closely related (<91%) to members of the order Chromatiales in the class Gammaproteobacteria. Phylogenetic analyses showed that this strain represented a distinct phylogenetic lineage in the order Chromatiales and could not be assigned to any of the defined families in the order. On the basis of low sequence similarities and differential characteristics of strain J89T from the genera of neighboring families, the strain is proposed to be a representative of a novel genus Aquichromatium gen. nov. A new family Aquichromatiaceae with the type genus Aquichromatium is proposed. Strain J89T (=MCCC 1K03281T=CMRC C2017206T) is the type strain of the type species Aquichromatium aeriopus sp. nov.

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References

  1. Arunasri K, Sasikala C, Ramana CV, Süling J, Imhoff JF (2005) Marichromatium indicum sp. nov., a novel purple sulfur gammaproteobacterium from mangrove soil of Goa, India. Int J Syst Evol Microbiol 55(2):673–679

    Article  CAS  PubMed  Google Scholar 

  2. Baek K, Choi A, Kang I, Im M, Cho J-C (2014) Granulosicoccus marinus sp. nov., isolated from Antarctic seawater, and emended description of the genus Granulosicoccus. Int J Syst Evol Microbiol 64(12):4103–4108

    Article  PubMed  Google Scholar 

  3. Biebl H, Drews G (1969) Das in vivo-Spektrum als taxonomisches Merkmal bei Untersuchungen zur Verbreitung von Athiorhodaceae. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg 123(4):425–452

    CAS  PubMed  Google Scholar 

  4. Bryantseva I, Gorlenko VM, Kompantseva EI, Imhoff JF, Süling J (1999) Thiorhodospira sibirica gen. nov., sp. nov., a new alkaliphilic purple sulfur bacterium from a Siberian soda lake. Int J Syst Evol Microbiol 49(2):697–703

    Google Scholar 

  5. Bryantseva I, Tourova T, Kovaleva O, Kostrikina N, Gorlenko V (2010) Ectothiorhodospira magna sp. nov., a new large alkaliphilic purple sulfur bacterium. Microbiology 79(6):780–790

    Article  CAS  Google Scholar 

  6. Collins M, Pirouz T, Goodfellow M, Minnikin D (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100(2):221–230

    Article  CAS  PubMed  Google Scholar 

  7. Dong X, Cai M (2001) Determinative manual for routine bacteriology. Scientific Press, Beijing (English translation)

    Google Scholar 

  8. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17(6):368–376

    Article  CAS  PubMed  Google Scholar 

  9. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783–791

    Article  PubMed  Google Scholar 

  10. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20(4):406–416

    Article  Google Scholar 

  11. Gerhardt P (1994) Methods for general and molecular bacteriology. Am Soc Microbiol, Washington DC

    Google Scholar 

  12. Gonzalez C, Gutierrez C, Ramirez C (1978) Halobacterium vallismortis sp. nov., an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24(6):710–715

    Article  CAS  PubMed  Google Scholar 

  13. Hallberg KB, Hedrich S, Johnson DB (2011) Acidiferrobacter thiooxydans gen. nov., sp. nov., an acidophilic, thermo-tolerant, facultatively anaerobic iron-and sulfur-oxidizer of the family Ectothiorhodospiraceae. Extremophiles 15(2):271–279

    Article  CAS  PubMed  Google Scholar 

  14. Imhoff JF (2005) Order Chromatiales

  15. Imhoff JF (2007) Family I. Chromatiaceae Bavendamm 1924, 125AL emend. Imhoff 1984b, 339. Bergey’s Manual® of Systematic Bacteriology: Volume 2: The Proteobacteria, Part B: The Gammaproteobacteria 2:3

  16. Imhoff JF (2007) Family II. Ectothiorhodospiraceae Imhoff 1984b, 339VP. Bergey’s Manual® of Systematic Bacteriology: Volume 2: The Proteobacteria, Part B: The Gammaproteobacteria 2:41

  17. Imhoff JF, Trüper HG (1977) Ectothiorhodospira halochloris sp. nov., a new extremely halophilic phototrophic bacterium containing bacteriochlorophyll b. Arch Microbiol 114(2):115–121

    Article  CAS  Google Scholar 

  18. Imhoff JF, Süling J, Petri R (1998) Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium. Int J Syst Evol Microbiol 48(4):1129–1143

    Google Scholar 

  19. Ivanova EP, Webb HK (2014) The family Granulosicoccaceae. In: the prokaryotes. Springer, pp 315-317

  20. Kelly D (2005) Family III. Halothiobacillaceae fam. nov. Kelly and Wood 2003. Bergey’s Man Syst Bacteriol 2:58–59

    Google Scholar 

  21. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62(3):716–721

    Article  CAS  PubMed  Google Scholar 

  22. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16(2):111–120

    Article  CAS  PubMed  Google Scholar 

  23. Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5(12):2359–2367

    Article  CAS  Google Scholar 

  24. Kurilenko VV, Christen R, Zhukova NV, Kalinovskaya NI, Mikhailov VV, Crawford RJ, Ivanova EP (2010) Granulosicoccus coccoides sp. nov., isolated from leaves of seagrass (Zostera marina). Int J Syst Evol Microbiol 60(4):972–976

    Article  PubMed  Google Scholar 

  25. Lee K, Lee HK, Choi T-H, Kim K-M, Cho J-C (2007) Granulosicoccaceae fam. nov., to include Granulosicoccus antarcticus gen. nov., sp. nov., a non-phototrophic, obligately aerobic chemoheterotroph in the order Chromatiales, isolated from Antarctic seawater. J Microbiol Biotechnol 17(9):1483–1490

    CAS  PubMed  Google Scholar 

  26. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57(7):1424–1428

    Article  PubMed  Google Scholar 

  27. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39(2):159–167

    Article  CAS  Google Scholar 

  28. Minnikin D, Collins M, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47(1):87–95

    Article  CAS  Google Scholar 

  29. Mori K, Suzuki K-i (2014) The family Thioalkalispiraceae. In: the prokaryotes. Springer, pp 653-658

  30. Mori K, Suzuki K-i, Urabe T, Sugihara M, Tanaka K, Hamada M, Hanada S (2011) Thioprofundum hispidum sp. nov., an obligately chemolithoautotrophic sulfur-oxidizing gammaproteobacterium isolated from the hydrothermal field on Suiyo Seamount, and proposal of Thioalkalispiraceae fam. nov. in the order Chromatiales. Int J Syst Evol Microbiol 61(10):2412–2418

    Article  CAS  PubMed  Google Scholar 

  31. Mori K, Suzuki K-i, Yamaguchi K, Urabe T, Hanada S (2015) Thiogranum longum gen. nov., sp. nov., an obligately chemolithoautotrophic, sulfur-oxidizing bacterium of the family Ectothiorhodospiraceae isolated from a deep-sea hydrothermal field, and an emended description of the genus Thiohalomonas. Int J Syst Evol Microbiol 65(1):235–241

    Article  CAS  PubMed  Google Scholar 

  32. Park S, Jung Y-T, Won S-M, Park J-M, Yoon J-H (2014) Granulosicoccus undariae sp. nov., a member of the family Granulosicoccaceae isolated from a brown algae reservoir and emended description of the genus Granulosicoccus. Antonie Van Leeuwenhoek 106(5):845–852

    Article  PubMed  Google Scholar 

  33. Rijkenberg MJ, Kort R, Hellingwerf KJ (2001) Alkalispirillum mobile gen. nov., spec. nov., an alkaliphilic non-phototrophic member of the Ectothiorhodospiraceae. Arch Microbiol 175(5):369–375

    Article  CAS  PubMed  Google Scholar 

  34. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425

    CAS  PubMed  Google Scholar 

  35. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids

  36. Schlesner H (1994) The development of media suitable for the microorganisms morphologically resembling Planctomyces spp., Pirellula spp., and other Planctomycetales from various aquatic habitats using dilute media. Syst Appl Microbiol 17(1):135–145

    Article  Google Scholar 

  37. Sorokin DY, Lysenko AM, Mityushina LL, Tourova TP, Jones BE, Rainey FA, Robertson LA, Kuenen GJ (2001) Thioalkalimicrobium aerophilum gen. nov., sp. nov. and Thioalkalimicrobium sibericum sp. nov., and Thioalkalivibrio versutus gen. nov., sp. nov., Thioalkalivibrio nitratis sp. nov., novel and Thioalkalivibrio denitrificancs sp. nov., novel obligately alkaliphilic and obligately chemolithoautotrophic sulfur-oxidizing bacteria from soda lakes. Int J Syst Evol Microbiol 51(2):565–580

    Article  CAS  PubMed  Google Scholar 

  38. Sorokin DY, Gorlenko VM, Tsapin AI, Nealson KH, Kuenen GJ (2002) Thioalkalimicrobium cyclicum sp. nov. and Thioalkalivibrio jannaschii sp. nov., novel species of haloalkaliphilic, obligately chemolithoautotrophic sulfur-oxidizing bacteria from hypersaline alkaline Mono Lake (California). Int J Syst Evol Microbiol 52(3):913–920

    CAS  PubMed  Google Scholar 

  39. Sorokin DY, Sjollema KA, Kuenen JG (2002) Thioalkalispira microaerophila gen. nov., sp. nov., a novel lithoautotrophic, sulfur-oxidizing bacterium from a soda lake. Int J Syst Evol Microbiol 52(6):2175–2182

    CAS  PubMed  Google Scholar 

  40. Sorokin DY, Tourova TP, Lysenko AM, Mityushina LL, Kuenen JG (2002) Thioalkalivibrio thiocyanoxidans sp. nov. and Thioalkalivibrio paradoxus sp. nov., novel alkaliphilic, obligately autotrophic, sulfur-oxidizing bacteria capable of growth on thiocyanate, from soda lakes. Int J Syst Evol Microbiol 52(2):657–664

    Article  CAS  PubMed  Google Scholar 

  41. Sorokin DY, Tourova TP, Bezsoudnova EY, Pol A, Muyzer G (2007) Denitrification in a binary culture and thiocyanate metabolism in Thiohalophilus thiocyanoxidans gen. nov. sp. nov.–a moderately halophilic chemolithoautotrophic sulfur-oxidizing Gammaproteobacterium from hypersaline lakes. Arch Microbiol 187(6):441–450

    Article  CAS  PubMed  Google Scholar 

  42. Sorokin DY, Muntyan MS, Panteleeva AN, Muyzer G (2012) Thioalkalivibrio sulfidiphilus sp. nov., a haloalkaliphilic, sulfur-oxidizing gammaproteobacterium from alkaline habitats. Int J Syst Evol Microbiol 62(8):1884–1889

    Article  CAS  PubMed  Google Scholar 

  43. Takai K, Miyazaki M, Hirayama H, Nakagawa S, Querellou J, Godfroy A (2009) Isolation and physiological characterization of two novel, piezophilic, thermophilic chemolithoautotrophs from a deep-sea hydrothermal vent chimney. Environ Microbiol 11(8):1983–1997

    Article  PubMed  Google Scholar 

  44. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  45. Venkata RV, Sasikala C, Veera VRE, Venkata RC (2010) Description of Ectothiorhodospira salini sp. nov. J Gen Appl Microbiol 56(4):313–319

    Article  Google Scholar 

  46. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu LH, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55(3):1149–1153

    Article  CAS  PubMed  Google Scholar 

  47. Yurkov VV, Krieger S, Stackebrandt E, Beatty JT (1999) Citromicrobium bathyomarinum, a novel aerobic bacterium isolated from deep-sea hydrothermal vent plume waters that contains photosynthetic pigment-protein complexes. J Bacteriol 181(15):4517–4525

    CAS  PubMed Central  PubMed  Google Scholar 

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Acknowledgements

This work was supported by projects funded under the National Basic Research Program of China (2013CB955700), the National Program on Global Change and Air–Sea Interaction (GASI-03-01-02-05), the National Natural Science Foundation of China (31600399), the China Postdoctoral Science Foundation (2016M592262, 2016M602566), the Key R&D projects in Shandong Province (2015GGF01014), the Natural Science Foundation of Shandong Province of China (ZR2016CB41), Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014), and CNOOC Zhanjiang Branch (CNOOC-KJ 125 FZDXM 00 ZJ 001-2014).

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Authors and Affiliations

  1. Research Center for Marine Biology and Carbon Sequestration, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, People’s Republic of China

    Liqiang Yang, Lili Tang & Yongyu Zhang

  2. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Lan Liu, Nimaichand Salam & Wen-Jun Li

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Lili Tang

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Yang, L., Tang, L., Liu, L. et al. Aquichromatium aeriopus gen. nov., sp. nov., A Non-phototrophic Aerobic Chemoheterotrophic Bacterium, and Proposal of Aquichromatiaceae fam. nov. in the Order Chromatiales . Curr Microbiol 74, 972–978 (2017). https://doi.org/10.1007/s00284-017-1275-1

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