Current Microbiology

, Volume 72, Issue 6, pp 795–803

Aeromonas lusitana sp. nov., Isolated from Untreated Water and Vegetables

  • Antonio Martínez-Murcia
  • Roxana Beaz-Hidalgo
  • Aaron Navarro
  • Mª. João Carvalho
  • Max Aravena-Román
  • Antonio Correia
  • Mª. José Figueras
  • Mª. José Saavedra
Review Article

Abstract

During previous studies to evaluate the phylogenetic diversity of Aeromonas from untreated waters and vegetables intended for human consumption, a group of isolates formed a unique gyrB phylogenetic cluster, separated from those of all other species described so far. A subsequent extensive phenotypic characterization, DNA–DNA hybridization, 16S rRNA gene sequencing, multi-locus phylogenetic analysis of the concatenated sequence of seven housekeeping genes (gyrB, rpoD, recA, dnaJ, gyrA, dnaX, and atpD; 4705 bp), and ERIC-PCR, were performed in an attempt to ascertain the taxonomy position of these isolates. This polyphasic approach confirmed that they belonged to a novel species of the genus Aeromonas, for which the name Aeromonas lusitana sp. nov. is proposed, with strain A.11/6T (=DSMZ 24095T, =CECT 7828T) as the type strain.

References

  1. 1.
    Abbott SL, Cheung WK, Janda JM (2003) The genus Aeromonas: biochemical characteristics, atypical reactions, and phenotypic identification schemes. J Clin Microbiol 41:2348–2357CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Allen DA, Austin B, Colwell RR (1983) Aeromonas media, a new species isolated from river water. Int J Syst Evol Microbiol 33:599–604Google Scholar
  3. 3.
    Alperi A, Martínez-Murcia AJ, Ko W-C, Monera A, Saavedra MJ, Figueras MJ (2010) Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., two new clinical species from Taiwan. Int J Syst Evol Microbiol 60:2048–2055CrossRefPubMedGoogle Scholar
  4. 4.
    Alperi A, Martínez-Murcia AJ, Monera A, Saavedra MJ, Figueras MJ (2010) Aeromonas fluvialis sp. nov., isolated from Spanish river. Int J Syst Evol Microbiol 60:72–77CrossRefPubMedGoogle Scholar
  5. 5.
    Aravena-Román M, Beaz-Hidalgo R, Inglis TJ, Riley TV, Martínez-Murcia AJ, Chang BJ, Figueras MJ (2013) Aeromonas australiensis sp. nov., isolated from irrigation water in Western Australia. Int J Syst Evol Microbiol 63:2270–2276CrossRefPubMedGoogle Scholar
  6. 6.
    Baumann P, Baumann L (1981) The marine gram negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes. In: Starr MP, Stolp H, Truper HG, Balows A, Schlegel HG (eds) The Prokaryotes, vol 2. Springer, Berlin, pp 1302–1331Google Scholar
  7. 7.
    Beaz-Hidalgo R, Alperi A, Figueras MJ, Romalde JL (2009) Aeromonas piscicola sp. nov., isolated from diseased fish. Syst Appl Microbiol 32:471–479CrossRefPubMedGoogle Scholar
  8. 8.
    Beaz-Hidalgo R, Martínez-Murcia AJ, Figueras MJRE et al (2013) Classification of Aeromonas hydrophila subsp. dhakensis Huys, 2002 and Aeromonas aquariorum Martínez-Murcia et al. 2008 as Aeromonas dhakensis sp. nov. comb nov. and emendation of the species Aeromonas hydrophila. Syst Appl Microbiol 36:171–176CrossRefPubMedGoogle Scholar
  9. 9.
    Beaz-Hidalgo R, Latif-Eugenin F, Hossain MJ, Berg K, Niemi RM, Rapala J, Lyra C, Liles MR, Figueras MJ (2015) Aeromonas aquatica sp. nov., Aeromonas finlandiensis sp. nov. and Aeromonas lacus sp. nov. isolated from Finnish waters associatedwith cyanobacterial blooms. Syst Appl Microbiol 38:161–168CrossRefPubMedGoogle Scholar
  10. 10.
    Carvalho MJ, Martínez-Murcia A, Esteves AC, Correia A, Saavedra MJ (2012) Phylogenetic diversity, antibiotic resistance and virulence traits of Aeromonas spp. from untreated waters for human consumption. Int J Food Microbiol 159:230–239CrossRefPubMedGoogle Scholar
  11. 11.
    Collado L, Cleenwerck I, Van Trappen S, de Vos P, Figueras MJ (2009) Arcobacter mytili sp. nov., an indoxyl acetate hydrolysis negative bacterium isolated from mussels. Int J Syst Evol Microbiol 59:1391–1396CrossRefPubMedGoogle Scholar
  12. 12.
    Demarta A, Küpfer M, Riegel P, Harf-Monteil C, Tonolla M, Peduzzi R, Monera A, Saavedra MJ, Martínez-Murcia AJ (2008) Aeromonas tecta sp. nov., isolated from clinical and environmental sources. Syst Appl Microbiol 46:439–444CrossRefGoogle Scholar
  13. 13.
    Esteve C, Gutiérrez MC, Ventosa A (1995) Aeromonas encheleia sp. nov., isolated from European eels. Int J Syst Evol Microbiol 45:462–466Google Scholar
  14. 14.
    Figueras MJ, Alperi A, Beaz-Hidalgo R, Stackebrandt E, Brambilla EM, Monera A, Martínez-Murcia AJ (2011) Aeromonas rivuli sp. nov., isolated from the upstream region of a karst water rivulet in Germany. Int J Syst Evol Microbiol 61:242–248CrossRefPubMedGoogle Scholar
  15. 15.
    Hänninem ML (1994) Phenotypic characteristics of the three hybridization groups of Aeromonas hydrophila complex isolated from different sources. J Appl Bacteriol 76:455–462CrossRefGoogle Scholar
  16. 16.
    Harf-Monteil C, Fleche AL, Riegel P, Prevost G, Bermond D, Grimont PA, Monteil H (2004) Aeromonas simiae sp. nov., isolated from monkey faeces. Int J Syst Evol Microbiol 54:481–485CrossRefPubMedGoogle Scholar
  17. 17.
    Haynes C (1951) Pseudomonas aeruginosa, its characterization and identification. J Gen Microbiol 5:939CrossRefPubMedGoogle Scholar
  18. 18.
    Huys G, Kämpfer P, Altwegg M, Coopman R, Janssen P, Gillis M, Kersters K (1997) Inclusion of Aeromonas DNA hybridization group 11 in Aeromonas encheleia and extended descriptions of the species Aeromonas eucrenophila and A. encheleia. Int J Syst Bacteriol 47:1157–1164CrossRefPubMedGoogle Scholar
  19. 19.
    Huys G, Kämpfer P, Albert MJ, Kühn I, Denys R, Swings J (2002) Aeromonas hydrophila subsp. dhakensis subsp. nov., isolated from children with diarrhoea in Bangladesh, and extended description of Aeromonas hydrophila subsp. hydrophila (Chester 1901) Stanier 1943 (approved lists 1980). Int J Syst Evol Microbiol 52:705–712PubMedGoogle Scholar
  20. 20.
    Janda JM, Abbott SL (2010) The genus Aeromonas, taxonomy, pathogenicity, and infection. Clin Microbiol Rev 23:35–73CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Janda JM, Bottone EJ (1981) Pseudomonas aeruginosa enzyme profiling: predictor of potential invasiveness and use as an epidemiological tool. J Clin Microbiol 14:55–60PubMedPubMedCentralGoogle Scholar
  22. 22.
    Janda JM, Abbott S, Khashe S, Kellogg GH, Shimada T (1996) Further Studies on biochemical characteristics and serolohgic properties of the genus Aeromonas. J Clin Microbiol 34:1930–1933PubMedPubMedCentralGoogle Scholar
  23. 23.
    Kitaura T, Doke S, Azuma I, Imaida M, Miyano K, Harada K, Yabuuchi E (1983) Halo production by sulfatase activity of Vibrio vulnificus and Vibrio cholerae O1 on a new selective sodium dodecyl sulphate-containing agar medium: a screening marker in environmental surveillance. FEMS Microbiol Lett 17:205–209CrossRefGoogle Scholar
  24. 24.
    MacFaddin JF (1993) Pruebas bioquímicas para la identificación de bacterias de importancia clínica. The William & William Company, Baltimore Translation by Médica Panamericana S.S Google Scholar
  25. 25.
    Martin-Carnahan A, Joseph SW (2005) Order XII. Aeromonadales ord. nov. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey´s Manual of Systematic Bacteriology, vol 2, 2nd edn. Springer, New York, pp 556–578CrossRefGoogle Scholar
  26. 26.
    Martínez-Murcia AJ, Benlloch S, Collins MD (1992) Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA–DNA hybridizations. Int J Syst Bacteriol 42:412–421CrossRefPubMedGoogle Scholar
  27. 27.
    Martínez-Murcia A, Beaz-Hidalgo R, Saavedra MJ, Figueras MJ, Sedlacek I (2013) Aeromonas cavernicola sp. nov., isolated from fresh water of a brook in a cavern. Curr Microbiol 66:197–204CrossRefPubMedGoogle Scholar
  28. 28.
    Martínez-Murcia AJ, Figueras MJ, Saavedra MJ, Stackebrandt E (2007) The recently proposed species Aeromonas sharmana sp. nov., isolate GPTSA-6T, is not a member of the genus Aeromonas. Int Microbiol 10:61–64PubMedGoogle Scholar
  29. 29.
    Martínez-Murcia AJ, Saavedra MJ, Mota VR, Maier T, Stackebrandt E, Cousin S (2008) Aeromonas aquariorum sp. nov., isolated from aquaria of ornamental fish. Int J Syst Evol Microbiol 58:1169–1175CrossRefPubMedGoogle Scholar
  30. 30.
    Martínez-Murcia A, Monera A, Alperi A, Figueras MJ, Saavedra MJ (2009) Phylogenetic evidence suggests that strains of A. hydrophila subsp. dhakensis belong to the species Aeromonas aquariorum sp. nov. Curr Microbiol 58:76–80CrossRefPubMedGoogle Scholar
  31. 31.
    Martínez-Murcia AJ, Monera A, Saavedra MJ, Oncina R, Lopez-Alvarez M, Lara E, Figueras MJ (2011) Multilocus phylogenetic analysis of the genus Aeromonas. Syst Appl Microbiol 34:189–199CrossRefPubMedGoogle Scholar
  32. 32.
    Miñana-Galbis D, Farfán M, Lorén JG, Fusté MC (2002) Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from environmental and clinical samples in Spain. J Appl Microbiol 93:420–430CrossRefPubMedGoogle Scholar
  33. 33.
    Miñana-Galbis D, Farfán M, Fusté MC, Lorén JG (2004) Aeromonas molluscorum sp. nov., isolated from bivalve molluscs. Int J Syst Evol Microbiol 54:2073–2078CrossRefPubMedGoogle Scholar
  34. 34.
    Miñana-Galbis D, Farfán M, Fusté MC, Lorén JG (2007) Aeromonas bivalvium sp. nov., isolated from bivalve molluscs. Int J Syst Evol Microbiol 57:582–587CrossRefPubMedGoogle Scholar
  35. 35.
    Miñana-Galbis D, Farfán M, Lorén JG, Fusté MC (2010) Proposal to assign Aeromonas diversa sp. nov. as a novel species designation for Aeromonas group 501. Syst Appl Microbiol 33:15–19CrossRefPubMedGoogle Scholar
  36. 36.
    Munson TE (1974) Improved KCN medium. Appl Microbiol 27:262–263PubMedPubMedCentralGoogle Scholar
  37. 37.
    Smibert RM, Krieg NR (1981) General characterization. In: Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Philips GB (eds) Manual of Methods for General Bacteriology. American Society for Microbiology, Washington, DC, pp 409–443Google Scholar
  38. 38.
    Stackebrandt E, Frederiksen W, Garrity GM, Grimont AD, Kämpfer P, Maiden MCJ, Nesme X, Roselló-Mora R, Swings J, Trüper HG, Vauterin L, Ward A, Whitman WB (2002) Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047PubMedGoogle Scholar
  39. 39.
    Urdiain M, López-López A, Gonzalo C, Busse HJ, Langer S, Kämpfer P, Rosselló-Móra R (2008) Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov., a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium, Rhodobium orientis and Rhodobium gokarnense. Syst Appl Microbiol 31:339–351CrossRefPubMedGoogle Scholar
  40. 40.
    Valera L, Esteve C (2002) Phenotypic study by numerical taxonomy of strains belonging to the genus Aeromonas. J Appl Microbiol 93:77–95CrossRefPubMedGoogle Scholar
  41. 41.
    Versalovic J, Koeuth T, Lupski JR (1991) Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19:6823–6831CrossRefPubMedPubMedCentralGoogle Scholar
  42. 42.
    Wayne LG, Brenner DJ, Colwell RR, Grimont PDA, Kandler O, Krichevsky ML, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Truper HG (1987) Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  43. 43.
    Ziemke F, Hofle MG, Lalucat J, Rosselló-Móra R (1998) Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Antonio Martínez-Murcia
    • 1
    • 2
  • Roxana Beaz-Hidalgo
    • 3
  • Aaron Navarro
    • 2
  • Mª. João Carvalho
    • 4
  • Max Aravena-Román
    • 5
  • Antonio Correia
    • 4
  • Mª. José Figueras
    • 3
  • Mª. José Saavedra
    • 6
  1. 1.Area de Microbiología, EPSOUniversidad Miguel HernándezOrihuelaSpain
  2. 2.Genetic PCR Solutions TMCEEIElcheSpain
  3. 3.Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, IISPVUniversitat Rovira i VirgiliReusSpain
  4. 4.Department of Biology & CESAMUniversity of AveiroAveiroPortugal
  5. 5.School of Pathology and Laboratory MedicineThe University of Western Australia CrawleyCrawleyAustralia
  6. 6.Department of Veterinary SciencesCECAV-University of Trás-os-Montes e Alto DouroVila RealPortugal

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