Abstract
Phage H188, a novel Vibrio kanaloae phage, was isolated from the surface water of Yellow Sea. Morphological analysis by transmission electron microscopy reveals that it belongs to the family Myoviridae. Present result suggests that the phage is stable at pH between 4.0 and 12.0. No significant difference in phage titers is noted at temperature 30-50 °C. A latent period of approximately 96 mins is indicated by the one-step growth curve. And, the burst size is about three virions per cell. Furthermore, genomic analysis of H188 reveals a genome size of 50364 bp with 43.63 % G+C content, and 76 putative open reading frames. There is no obvious similarity between H188 and other known phages by genomic comparison. Moreover, the H188 genome includes modules for phage structure, phage packaging, DNA replication and regulation, and some additional functions.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (NSFC) (Nos. 41076088, 31500339), the National Key Basic Research Program of China (973 Program) (No. 2013CB429704), the China Postdoctoral Science Foundation (No. 2015M570612), and the Chinese Polar Environment comprehensive Investigation & Assessment Programs (No. CHINARE-01-05).
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Li, Y., Wang, M., Liu, Q. et al. Complete Genomic Sequence of Bacteriophage H188: A Novel Vibrio kanaloae Phage Isolated from Yellow Sea. Curr Microbiol 72, 628–633 (2016). https://doi.org/10.1007/s00284-015-0984-6
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DOI: https://doi.org/10.1007/s00284-015-0984-6