Skip to main content
SpringerLink
Log in

Strawberry Fruit Rot Caused by Neopestalotiopsis iranensis sp. nov., and N. mesopotamica

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

A new species of Neopestalotiopsis based on both morphological and molecular characteristics is described. Neopestalotiopsis iranensis sp. nov. isolated from rotted strawberry (Fragaria ananassa) fruits as well as from stolon and leaf lesions in Kurdistan province, Iran. Initially, light tan and sunken spots developed on fruits and resulted in a soft decay of the fruit flesh. The new species is morphologically distinguished from similar species with different conidium size and by possessing longer apical appendages, as well as some knobbed basal appendages. Phylogenetic analyses (Bayesian inference, maximum likelihood and maximum parsimony analyses) based on internal transcribed spacer, β-tubulin, and partial translation elongation factor 1-alpha combined gene sequences also indicated that this species is phylogenetically distinct from others. Moreover, strawberry crop is introduced here as a new host for N. mesopotamica.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Arzanlou M, Torbati M, Khodaei S, Bakhshi M (2012) Contribution to the knowledge of Pestalotioid fungi of Iran. Mycosphere 3:871–878. doi:10.5943/mycosphere/3/5/12.871

    Article  Google Scholar 

  2. Ayoubi N, Soleimani MJ (2013) Occurrence of Strawberry anthracnose disease caused by Colletotrichum acutatum in Kurdistan province. In: 1st Iranian Mycological Congress, 3–5 September, University of Guilan, Rasht, p 97

  3. Bose S (1970) Diseases of valley fruits in Kumaon (III). Leaf-spot diseases of strawberry. Progress Hortic 2:33–35

    Google Scholar 

  4. Camili EC, Carbonari M, de Souza NL (2002) Characterization of Pestalotiopsis longisetula and its pathogenicity in strawberry. Summa Phytopathol 28(2):213–214

    Google Scholar 

  5. Das R, Chutia M, Das K, Jha D (2010) Factors affecting sporulation of Pestalotiopsis disseminata causing grey blight disease of Persea bombycina Kost., the primary food plant of muga silkworm. Crop Prot 29:963–968. doi:10.1016/j.cropro.2010.05.012

    Article  Google Scholar 

  6. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  7. Embaby E (2007) Pestalotia fruit rot on strawberry plants in Egypt. Egypt J Phytopathol 35:99–110

    Google Scholar 

  8. Guba EF (1961) Monograph of monochaeta and pestalotia. Harvard University Press, Cambridge

    Google Scholar 

  9. Howard C, Albregts E (1973) Strawberry fruit rot caused by Pestalotia longisetula. Phytopathology 63:862–863

    Article  Google Scholar 

  10. Hu H, Jeewon R, Zhou D, Zhou T, Hyde KD (2007) Phylogenetic diversity of endophytic Pestalotiopsis species in Pinus armandii and Ribes spp.: evidence from rDNA and β-tubulin gene phylogenies. Fungal Divers 24:1–22

    CAS  Google Scholar 

  11. Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    Article  CAS  PubMed  Google Scholar 

  12. Keith LM, Velasquez ME, Zee FT (2006) Identification and characterization of Pestalotiopsis spp. causing scab disease of guava, Psidium guajava, in Hawaii. Plant Dis 90:16–23

    Article  CAS  Google Scholar 

  13. Ling X, Kusakari S, Hosomi A, Toyoda H, Ouchi S (1999) Postharvest disease of grape caused by Pestalotiopsis species. Ann Phytopathol Soc Jpn 65:305–311

    Article  Google Scholar 

  14. Maharachchikumbura SS, Chukeatirote E, Guo L-D, Crous PW, Mckenzie EH, Hyde KD (2013) Pestalotiopsis species associated with Camellia sinensis (tea). Mycotaxon 123:47–61

    Article  Google Scholar 

  15. Maharachchikumbura SS, Guo LD, Cai L, Chukeatirote E, Wu WP, Sun X, Crous PW, Bhat DJ, McKenzie EH, Bahkali AH, Hyde KD (2012) A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species. Fungal Divers 56:95–129. doi:10.1007/s13225-012-0198-1

    Article  Google Scholar 

  16. Maharachchikumbura SS, Guo LD, Chukeatirote E, Bahkali AH, Hyde KD (2011) Pestalotiopsis—morphology, phylogeny, biochemistry and diversity. Fungal Divers 50:167–187

    Article  Google Scholar 

  17. Maharachchikumbura SS, Guo LD, Chukeatirote E, Mckenzie EH, Hyde KD (2013) A destructive new disease of Syzygium samarangense in Thailand caused by the new species Pestalotiopsis samarangensis. Tropical Plant Pathol 38:227–235. doi:10.1590/S1982-56762013005000002

    Article  Google Scholar 

  18. Maharachchikumbura SS, Hyde KD, Groenewald JZ, Xu J, Crous PW (2014) Pestalotiopsis revisited. Stud Mycol 79:121–186

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala

    Google Scholar 

  20. Page RDM (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    CAS  PubMed  Google Scholar 

  21. Royse D, Ries S (1976) A cortical decay of petioles and stolons of strawberry caused by Pestalotia longisetula (Fungal diseases). Plant Dis Rep 60:901–902

    Google Scholar 

  22. Ronquist FM, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542. doi:10.1093/sysbio/sys029

    Article  PubMed Central  PubMed  Google Scholar 

  23. Shitole D, Patil U, Pawar N (2000) In vitro evaluation of chemicals and antibiotics against important fruit rotting fungi of strawberry. J Maharashtra Agric Univ 25:179–181

    Google Scholar 

  24. Song Y, Geng K, Zhang B, Hyde KD, Zhao WS, Wei JG, Kang JC, Wang Y (2013) Two new species of Pestalotiopsis from Southern China. Phytotaxa 126(1):22–30

    Article  Google Scholar 

  25. Sutton BC (1980) The Coelomycetes. Fungi imperfecti with pycnidia, acervuli and stromata. Commonwealth Mycological Institute, Kew

    Google Scholar 

  26. Swofford DL (2003) {PAUP*. Phylogenetic analysis using parsimony (* and other methods). Version 4.}

  27. Stamatakis A, Ludwig T, Meier H (2005) RAxML-III: a fast program for maximum likelihood-based inference of large phylogenetic trees. Bioinformatics 21:456–463

    Article  CAS  PubMed  Google Scholar 

  28. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 25:4876–4882

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Wei JG, Xu T (2004) Pestalotiopsis kunmingensis sp. nov., an endophyte from Podocarpus macrophyllus. Fungal Divers 15:247–254

    Google Scholar 

  30. Yasuda F, Kobayashi T, Watanabe H, Izawa H (2003) Addition of Pestalotiopsis spp. to leaf spot pathogens of Japanese persimmon. J Gen Plant Pathol 69:29–32

    Article  Google Scholar 

  31. Zhang Y, Maharachchikumbura SS, Mckenzie EH, Hyde KD (2012) A novel species of Pestalotiopsis causing leaf spots of Trachycarpus fortunei. Cryptogam Mycol 33:311–318. doi:10.7872/crym.v33.iss3.2012.311

    Article  Google Scholar 

Download references

Acknowledgments

Partial financial support for this work by a research grant (No. 32-996) from the Research Council of Bu-Ali Sina University, Hamedan, Iran, is gratefully acknowledged. We also wish to thank Dr. D. Zafari, Dept. Plant Protection, Bu-Ali Sina University, for facilitating the molecular work.

Author information

Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Bu-Ali Sina University, Mahdyeh St., Hamedan, 65174, Islamic Republic of Iran

    Najmeh Ayoubi & Mohammad J. Soleimani

Authors
  1. Najmeh Ayoubi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mohammad J. Soleimani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohammad J. Soleimani.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ayoubi, N., Soleimani, M.J. Strawberry Fruit Rot Caused by Neopestalotiopsis iranensis sp. nov., and N. mesopotamica . Curr Microbiol 72, 329–336 (2016). https://doi.org/10.1007/s00284-015-0955-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-015-0955-y

Keywords

Search

Navigation