Abstract
We isolated and purified a novel virulent Pseudoalteromonas bacteriophage PHq0 and the host bacterium Pseudoalteromonas BQ0 from seawater collected in a coastal area of the Yellow Sea of China. (36°06′N, 120°32′E). Transmission electron microscopy revealed that the phage had an icosahedral head of 50 nm in diameter with a long tail of 100 nm. The one-step growth curve showed the latent period of about 15 min, a rise period of 15 min, and a burst size of about 363 virions. The genome of phage PHq0 was found to consist of a linear, double-stranded 33,399-bp DNA molecule with a GC content of 40.29 % and 56 putative open reading frames (ORFs). Among these genes, 23 conserved domains were detected by BLASTP, 17 were functionally known, leaving 39 unknown putative genes, BLASTP results show that 57.14 % of the 56 predicted ORFs were not found to have any matches of putative functions or conserved domains in the BLASTP database which should be classified as a new member of the Siphoviridae family. The phage PHq0 genome adds a new Siphoviridae-family phage genome for marine bacteriophages which will provide useful basic information for further molecular research on interaction mechanism between bacteriophages and their hosts.
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Acknowledgments
We are thankful to Professor Zhang Xiao-Hua, Microbiology lab, College of Marine Life, Ocean University of China, for providing bacterial strains to carry out phage host-range experiments and suggestions during the preparation of this manuscript. We are grateful to the research vessel Dong Fang Hong 2, for providing the seawater samples. The research was funded by the National Natural Science Foundation of China (No. 41076088) and China Postdoctoral Science Foundation, No.2015M5Z0612.
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Wang, Db., Li, Y., Sun, Mq. et al. Complete Genome of a Novel Pseudoalteromonas Phage PHq0. Curr Microbiol 72, 81–87 (2016). https://doi.org/10.1007/s00284-015-0919-2
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DOI: https://doi.org/10.1007/s00284-015-0919-2