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Mycobacterium smegmatis MSMEG_3705 Encodes a Selective Major Facilitator Superfamily Efflux Pump with Multiple Roles

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Abstract

Mycobacterium smegmatis mc2155 MSMEG_3705 gene was annotated to encode a transporter protein that contains 12 alpha-helical transmembrane domains. We predicted MSMEG_3705 encoding a major facilitator superfamily (MFS) member. To confirm the prediction, the M. smegmatis mc2155 MSMEG_3705 gene was deleted. The MSMEG_3705 deletion mutant strain M. smegmatis mc2155 ∆MSMEG_3705 was more sensitive to capreomycin. Moreover, M. smegmatis mc2155 ∆MSMEG_3705 strain accumulated more ethidium bromide intracellular than wild-type M. smegmatis mc2155. Quite unexpectedly, M. smegmatis mc2155 ∆MSMEG_3705 grew faster than the wild-type M. smegmatis mc2155. The upregulation of the expression of MSMEG_3706, a gene encoding isocitrate lyase downstream MSMEG_3705, in the deletion mutant, might underlie such faster growth in the mutant. The study showed that MSMEG_3705 encodes a genuine MFS member and plays significant role in bacterial growth and antibiotics resistance.

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Acknowledgments

This work is supported by National Natural Science Foundation (Grant Numbers 81371851, 81071316, 81271882, 81301394), New Century Excellent Talents in Universities (Grant Number NCET-11-0703), National Megaprojects for Key Infectious Diseases (Grant Number 2008ZX10003-006), Excellent PhD thesis fellowship of Southwest University (Grant Numbers kb2010017, ky2011003), the Fundamental Research Funds for the Central Universities (Nos. XDJK2011D006, XDJK2012D011, XDJK2012D007, XDJK2013D003 and XDJK2014D040), Natural Science Foundation Project of CQ CSTC (Grant Number CSTC 2010BB5002), The Chongqing municipal committee of Education for postgraduates excellence program (No.YJG123104), and the undergraduates teaching reform program (No. 2013JY201).

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Authors and Affiliations

  1. Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Three Gorges Eco-environment and Bioresources, Key Laboratory Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, 400715, China

    Zhen Zhang, Rui Wang & Jianping Xie

  2. Chongqing Center for Clinical Laboratory, Yuzhong, Chongqing, China

    Zhen Zhang

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  1. Zhen Zhang
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  2. Rui Wang
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  3. Jianping Xie
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Correspondence to Jianping Xie.

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Zhang, Z., Wang, R. & Xie, J. Mycobacterium smegmatis MSMEG_3705 Encodes a Selective Major Facilitator Superfamily Efflux Pump with Multiple Roles. Curr Microbiol 70, 801–809 (2015). https://doi.org/10.1007/s00284-015-0783-0

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  • DOI: https://doi.org/10.1007/s00284-015-0783-0

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