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Important Role of Catalase in the Cellular Response of the Budding Yeast Saccharomyces cerevisiae Exposed to Ionizing Radiation

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Abstract

Ionizing radiation indirectly causes oxidative stress in cells via reactive oxygen species (ROS), such as hydroxyl radicals (OH−) generated by the radiolysis of water. We investigated how the catalase function was affected by ionizing radiation and analyzed the phenotype of mutants with a disrupted catalase gene in Saccharomyces cerevisiae exposed to radiation. The wild-type yeast strain and isogenic mutants with disrupted catalase genes were exposed to various doses of 60Co gamma-rays. There was no difference between the wild-type strain and the cta1 disruption mutant following exposure to gamma-ray irradiation. In contrast, there was a significant decrease in the ctt1 disruption mutant, suggesting that this strain exhibited decreased survival on gamma-ray exposure compared with other strains. In all three strains, stationary phase cells were more tolerant to the exposure of gamma-rays than exponential phase cells, whereas the catalase activity in the wild-type strain and cta1 disruption mutant was higher in the stationary phase than in the exponential phase. These data suggest a correlation between catalase activity and survival following gamma-ray exposure. However, this correlation was not clear in the ctt1 disruption mutant, suggesting that other factors are involved in the tolerance to ROS induced by irradiation.

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Authors and Affiliations

  1. Division of Applied Life Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, Japan

    Takuto Nishimoto, Michihiko Kataoka & Masao Kishida

  2. Department of Quantum and Radiation, Graduate School of Technology, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, Japan

    Masakazu Furuta

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  1. Takuto Nishimoto
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  2. Masakazu Furuta
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  3. Michihiko Kataoka
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  4. Masao Kishida
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Correspondence to Masao Kishida.

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Nishimoto, T., Furuta, M., Kataoka, M. et al. Important Role of Catalase in the Cellular Response of the Budding Yeast Saccharomyces cerevisiae Exposed to Ionizing Radiation. Curr Microbiol 70, 404–407 (2015). https://doi.org/10.1007/s00284-014-0733-2

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  • DOI: https://doi.org/10.1007/s00284-014-0733-2

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