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Characterization of Fungi from Ruminal Fluid of Beef Cattle with Different Ages and Raised in Tropical Lignified Pastures

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Abstract

The objective of this study was to evaluate the aerobic rumen mycobiota from three age groups of Nelore beef cattle reared extensively on lignified pasture. The experiment was randomized and sampled 50 steers, 50 cows, and 50 calves grazed on Brachiaria spp. pasture during the dry season. Rumen fluid in all animals was aromatic, slightly viscous, and greenish-brown in color. Microscopic examination revealed monocentric and polycentric anaerobic fungi in similar proportions (P > 0.05) in the rumen fluid of cows and steers. However, these microorganisms were not identified in any of the samples from calves. In culture exams, aerobic filamentous population was significantly higher for rumen fluid of cows compared to the other two groups. Microculture and rDNA sequence analyses showed Aspergillus spp. as the most frequent aerobic fungus among the isolates from the three bovine groups evaluated. Biochemical profiles were determined by the growth level of yeast isolates with 44 nutrient sources. Ten different yeast profiles were obtained, and yeast isolates from cow ruminal fluid showed ability to catabolize greater diversity of carbon and nitrogen sources. The differences in the fungal populations observed in this study could be explained by microbial and physiological interactions existing in the ruminal ecosystem of each age bovine group. The present study showed the fungal population of the rumen related with differences among age of cattle raised in lignified pastures. Metabolic capabilities of mycelial fungi or yeast identified in this study may be employed in new probiotics or microbial additives for different bovine categories.

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Abbreviations

ADF:

Acid detergent fiber

CFU:

Colony forming units

CP:

Crude protein

DM:

Dry matter

EE:

Ether extract

NDF:

Neutral detergent fiber

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Acknowledgments

This work was supported by CAPES (Coordination of Improvement of Higher Education Personnel), National Council for Scientific and Technological Development (CNPq), Foundation for Research Support of the State of Minas Gerais (FAPEMIG) and Pro-rector of research of the Federal University of Minas Gerais.

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Author notes

  1. Eduardo Robson Duarte

    Present address: Avenida Universitária, 1.000, Bairro Universitário, Montes Claros, MG, 39404-006, Brazil

Authors and Affiliations

  1. Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, Montes Claros, MG, 39400-401, Brazil

    Eduardo Robson Duarte, Cláudio Eduardo Silva Freitas, Edvaldo Alves Vieira & Luciana Castro Geraseev

  2. Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil

    Alice Ferreira da Silva-Hughes & Carlos Augusto Rosa

  3. Escola de Medicina Veterinária, Universidade Federal de Minas Gerais, 39400-401, Montes Claros, MG, Brazil

    Flávia Oliveira Abrão & Norberto Mario Rodrigues

Authors
  1. Flávia Oliveira Abrão
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  2. Eduardo Robson Duarte
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  3. Cláudio Eduardo Silva Freitas
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Correspondence to Eduardo Robson Duarte.

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Abrão, F.O., Duarte, E.R., Freitas, C.E.S. et al. Characterization of Fungi from Ruminal Fluid of Beef Cattle with Different Ages and Raised in Tropical Lignified Pastures. Curr Microbiol 69, 649–659 (2014). https://doi.org/10.1007/s00284-014-0633-5

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  • DOI: https://doi.org/10.1007/s00284-014-0633-5

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