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Potential of Siderophore Production by Bacteria Isolated from Heavy Metal: Polluted and Rhizosphere Soils

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Abstract

Recently, heavy metals have been shown to have a stimulating effect on siderophore biosynthesis in various bacteria. In addition, several studies have found that siderophore production is greater in bacteria isolated from soil near plant roots. The aim of this study was to compare the production of siderophores by bacterial strains isolated from heavy metal-contaminated and uncontaminated soils. Chrome azurol sulphonate was used to detect siderophore secretion by several bacterial strains isolated from heavy metal-contaminated and rhizosphere-uncontaminated soils with both a qualitative disc diffusion method and a quantitative ultraviolet spectrophotometric method. Siderophore production by rhizosphere bacteria was significantly greater than by bacteria isolated from contaminated soil. The Pearson’s correlation test indicated a positive correlation between the amount of siderophore produced by bacteria isolated from the rhizosphere using the quantitative and qualitative detection methods and the amount of heavy metal in the soil. However, a significant negative correlation was observed between the amount of siderophore produced by bacteria isolated from heavy metal-contaminated soil and the amount of heavy metal (r value of −0.775, P < 0.001).

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Acknowledgments

This study was supported by the National Research Foundation of Korea Grant funded by the Korean government (Project number: NRF-2013R1A1A2011950) and also supported by Kangwon National University.

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  1. Department of Biological Environment, Kangwon National University, 192-1, Hyoja 2-dong, Chuncheon, Kangwon-do, 200-701, Republic of Korea

    Khalid A. Hussein & Jin Ho Joo

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  1. Khalid A. Hussein
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  2. Jin Ho Joo
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Correspondence to Jin Ho Joo.

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Hussein, K.A., Joo, J.H. Potential of Siderophore Production by Bacteria Isolated from Heavy Metal: Polluted and Rhizosphere Soils. Curr Microbiol 68, 717–723 (2014). https://doi.org/10.1007/s00284-014-0530-y

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  • DOI: https://doi.org/10.1007/s00284-014-0530-y

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