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Development of SCAR Markers to Determine the Mating Types of Lepista nuda Protoplast Monokaryons

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Abstract

Lepista nuda (Bull. ex Fr.) Cooke belongs to Tricholomataceae and is an edible fungus with both economic and medical value. Mycelia were isolated from the fruiting bodies of L. nuda and were used to prepare the protoplast monokaryons. One hundred and fifteen monokaryons were obtained and their mating types were determined using somatic incompatibility tests. Protoplast monokaryons segregated into either the A1B1 or the A2B2 mating types. Inter-simple sequence repeats and sequence-related amplified polymorphism fingerprinting were used to analyse the mating types of these protoplast monokaryons and 16 sequence-characterised amplified region primers were developed to efficiently differentiate between the monokaryon mating types. Multiplex PCR analyses were also established. The data presented here outline a method for the precise and rapid identification of protoplast monokaryon mating types, which has the promise to shorten the period required for conventional crossbreeding.

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Acknowledgments

Thanks to the anonymous reviewers for improvement on this paper. Thanks to Prof. Xinghong Li and Dr. Jiye Yan for providing Lepista nuda strain. This work was supported by Grants from the Beijing Nova Program (Grant No. 2011053) and Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Science (Grant No. CCJJZDXM201201-3)

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, 100097, China

    Dengjin Li, Yu Liu, Peng Wang, Yuanwei Ma, Shouxian Wang, Shuang Zhao & Feng Xu

  2. University of Chinese Academy of Science, Beijing, 100049, China

    Dengjin Li

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  1. Dengjin Li
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  2. Yu Liu
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  3. Peng Wang
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  4. Yuanwei Ma
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  5. Shouxian Wang
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  6. Shuang Zhao
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  7. Feng Xu
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Correspondence to Feng Xu.

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Li, D., Liu, Y., Wang, P. et al. Development of SCAR Markers to Determine the Mating Types of Lepista nuda Protoplast Monokaryons. Curr Microbiol 68, 536–542 (2014). https://doi.org/10.1007/s00284-013-0510-7

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  • DOI: https://doi.org/10.1007/s00284-013-0510-7

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