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Use of the IRAP Marker to Study Genetic Variability in Pseudocercospora fijiensis Populations

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Abstract

Pseudocercospora fijiensis is the etiological agent of black Sigatoka, which is currently considered as one of the most destructive banana diseases in all locations where it occurs. It is estimated that a large portion of the P. fijiensis genome consists of transposable elements, which allows researchers to use transposon-based molecular markers in the analysis of genetic variability in populations of this pathogen. In this context, the inter-retrotransposon-amplified polymorphism (IRAP) was used to study the genetic variability in P. fijiensis populations from different hosts and different geographical origins in Brazil. A total of 22 loci were amplified and 77.3 % showed a polymorphism. Cluster analysis revealed two major groups in Brazil. The observed genetic diversity (H E) was 0.22, and through molecular analysis of variance, it was determined that the greatest genetic variability occurs within populations. The discriminant analysis of principal components revealed no structuring related to the geographical origin of culture of the host. The IRAP-based marker system is a suitable tool for the study of genetic variability in P. fijiensis.

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Acknowledgments

This work was financially supported by the Brazilian Agency CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), and FAPEMIG (Fundação de Amparo à Pesquisa do estado de Minas Gerais).

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Authors and Affiliations

  1. Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil

    Casley Borges de Queiroz, Mateus Ferreira Santana, Elza Fernandes de Araújo & Marisa Vieira de Queiroz

  2. Laboratório de Genética de Micro-organismo, Embrapa Amazônia Ocidental, Manaus, Amazonas, Brazil

    Gilvan Ferreira da Silva

  3. Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil

    Eduardo Seiti Gomide Mizubuti

Authors
  1. Casley Borges de Queiroz
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  2. Mateus Ferreira Santana
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  3. Gilvan Ferreira da Silva
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  4. Eduardo Seiti Gomide Mizubuti
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  5. Elza Fernandes de Araújo
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  6. Marisa Vieira de Queiroz
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Correspondence to Marisa Vieira de Queiroz.

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de Queiroz, C.B., Santana, M.F., da Silva, G.F. et al. Use of the IRAP Marker to Study Genetic Variability in Pseudocercospora fijiensis Populations. Curr Microbiol 68, 358–364 (2014). https://doi.org/10.1007/s00284-013-0454-y

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  • DOI: https://doi.org/10.1007/s00284-013-0454-y

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