Abstract
Food borne diseases have a major impact on public health whose epidemiology is rapidly changing. The whole cells of pathogens involved or their toxins/metabolites affect the human health apart from spoiling sensory properties of the food products finally affecting the food industry as well as consumer health. With pathogens developing mechanisms of antibiotic resistance, there has been an increased need to replace antibiotics as well as chemical additives with naturally occurring bacteriocins. Bacteriocins are known to act mainly against Gram-positive pathogens and with little or no effect towards Gram-negative enteric bacteria. In the present study, combination effect of lipase and bacteriocin produced by Enterococcus faecium NCIM5363, a highly lipolytic lactic acid bacterium against various food pathogens was assessed. The lipase in combination with enterocin exhibited a lethal effect against Gram-negative pathogens. Scanning electron microscopy studies carried out to ascertain the constitutive mode of action of lipase and enterocin revealed that the lipase degrades the cell wall of Gram-negative bacteria and creates a pore through which enterocin enters thereby resulting in cell death. The novelty of this work is the fact that this is the first report revealing the synergistic effect of lipase with enterocin against Gram-negative bacteria.
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Acknowledgments
Authors thank their parent organization Council of Scientific and Industrial Research (CSIR) for funding this work through Encouraging & Motivating World class Exploratory Research (EMPOWER) scheme to NB. VR thanks University Grants Commission (UGC), Govt. of India for the senior research fellowship. Authors place on record their thanks to Director, CFTRI for encouragement and permission to publish the work.
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Ramakrishnan, V., Narayan, B. & Halami, P.M. Combined Effect of Enterocin and Lipase From Enterococcus faecium NCIM5363 Against Food Borne Pathogens: Mode of Action Studies. Curr Microbiol 65, 162–169 (2012). https://doi.org/10.1007/s00284-012-0138-z
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DOI: https://doi.org/10.1007/s00284-012-0138-z