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Display of Bombyx mori Nucleopolyhedrovirus GP64 on the Bacillus subtilis Spore Coat

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Abstract

To investigate whether Bombyx mori immunized with Bacillus subtilis spore displaying GP64 escape from the B. mori nucleopolyhedrovirus (BmNPV) attack, a recombinant integrative plasmid named pJS700-GP64 was constructed, which carries a recombinant cotC-Gp64 gene under the control of the cotC promoter. In this study, pJS700-GP64 was transformed into B. subtilis 168 (trp−) competent cells, an amylase (amyE) inactivated mutant was selected, and was confirmed to be a double cross-over integrant, cotC-Gp64 fragment of which was integrated into B. subtilis chromosome. Gp64 was expressed on the spore surface and recognized by Gp64-specific antibody. Results of B. mori when challenged with BmNPV indicated that B. mori vaccinated with the recombinant spores possessed resistance to the invasion of BmNPV at some degree.

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Acknowledgments

This article was supported by the National Natural Science Foundation of China (31000080), the grants from Jiangsu Sci-Tech Support Project-Agriculture (No. BE2008379), and Scientific Research Starting Fund projects for senior professionals (09JDG057), from Jiangsu University.

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Authors and Affiliations

  1. Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, China

    Guohui Li, Qi Tang, Huiqing Chen, Qin Yao & Keping Chen

  2. School of Environment, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, China

    Degang Ning

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  1. Guohui Li
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  2. Qi Tang
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  3. Huiqing Chen
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  4. Qin Yao
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  5. Degang Ning
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  6. Keping Chen
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Corresponding author

Correspondence to Keping Chen.

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Guohui Li and Qi Tang have contributed equally to this study.

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Li, G., Tang, Q., Chen, H. et al. Display of Bombyx mori Nucleopolyhedrovirus GP64 on the Bacillus subtilis Spore Coat. Curr Microbiol 62, 1368–1373 (2011). https://doi.org/10.1007/s00284-011-9867-7

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  • DOI: https://doi.org/10.1007/s00284-011-9867-7

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