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Desulfovibrio legallis sp. nov.: A Moderately Halophilic, Sulfate-Reducing Bacterium Isolated from a Wastewater Digestor in Tunisia

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Abstract

A new moderately halophilic sulfate-reducing bacterium (strain H T1 ) was enriched and isolated from a wastewater digestor in Tunisia. Cells were curved, motile rods (2–3 x 0.5 μm). Strain H T1 grew at temperatures between 22 and 43°C (optimum 35°C), and at pH between 5.0 and 9.2 (optimum 7.3–7.5). Strain H T1 required salt for growth (1–45 g of NaCl/l), with an optimum at 20–30 g/l. Sulfate, sulfite, thiosulfate, and elemental sulfur were used as terminal electron acceptors but not nitrate and nitrite. Strain H T1 utilized lactate, pyruvate, succinate, fumarate, ethanol, and hydrogen (in the presence of acetate and CO2) as electron donors in the presence of sulfate as electron acceptor. The main end-products from lactate oxidation were acetate with H2 and CO2. The G + C content of the genomic DNA was 55%. The predominant fatty acids of strain H T1 were C15:0 iso (38.8%), C16:0 (19%), and C14:0 iso 3OH (12.2%), and menaquinone MK-6 was the major respiratory quinone. Phylogenetic analysis of the small-subunit (SSU) ribosomal RNA (rRNA) gene sequence indicated that strain H T1 was affiliated to the genus Desulfovibrio. On the basis of SSU rRNA gene sequence comparisons and physiological characteristics, strain H T1 is proposed to be assigned to a novel species of sulfate reducers of the genus Desulfovibrio, Desulfovibrio legallis sp. nov. (= DSM 19129T = CCUG 54389T).

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Authors and Affiliations

  1. INSAT, Laboratoire d’Ecologie et de Technologie Microbienne, Centre Urbain Nord, BP 676, 1080, Tunis Cedex, Tunisia

    Olfa Ben Dhia Thabet, Terres Wafa, Khelifi Eltaief & Moktar Hamdi

  2. Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, Case 925, 163 Avenue de Luminy, F-13288, Marseille Cedex 9, France

    Jean-Luc Cayol, Guy Fauque & Marie-Laure Fardeau

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  1. Olfa Ben Dhia Thabet
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  2. Terres Wafa
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  3. Khelifi Eltaief
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  4. Jean-Luc Cayol
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  5. Moktar Hamdi
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  6. Guy Fauque
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  7. Marie-Laure Fardeau
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Correspondence to Marie-Laure Fardeau.

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Thabet, O.B.D., Wafa, T., Eltaief, K. et al. Desulfovibrio legallis sp. nov.: A Moderately Halophilic, Sulfate-Reducing Bacterium Isolated from a Wastewater Digestor in Tunisia. Curr Microbiol 62, 486–491 (2011). https://doi.org/10.1007/s00284-010-9733-z

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  • DOI: https://doi.org/10.1007/s00284-010-9733-z

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