Abstract
Helicobacter pylori (Hp) is a major risk factor for gastrointestinal disorders including gastric cancer. We evaluated host serum antibody responses toward outer membrane protein18 in comparison with Urease A and B subunits. omp18 and ureA-ureB gene fragments were PCR amplified, cloned, and expressed in E. coli expression system. The expressed proteins were visualized on SDS-PAGE and confirmed by immuno-blotting. Purified proteins were applied in western blotting assays in comparison with local and foreign ELISA kits. ROC curve analysis identified the optimum cut-off points for each protein. rOmp18 represented the highest rates of sensitivity (94%), specificity (89%), PPV (97.4%), NPV (77.4%), and accuracy (93.2%) in comparison with urease A and B subunits. These immunologic indices were in “substantial” agreement (Κ = 0.7) with the gold standard tests for Hp detection. This study recommends Hp conserved Omp18 as a reliable serologic marker for accurate detection of Hp infection particularly for application in population screening approaches.
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This study was co-funded by a grant from Pasteur Institute of Iran in support of Ph.D. dissertations and a technical assistance grant from Islamic Development Bank, Jeddah, Saudi Arabia.
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Talebkhan, Y., Ebrahimzadeh, F., Esmaeili, M. et al. Helicobacter pylori Omp18 and Its Application in Serologic Screening of Infection. Curr Microbiol 62, 325–330 (2011). https://doi.org/10.1007/s00284-010-9694-2
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DOI: https://doi.org/10.1007/s00284-010-9694-2