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Lactobacillus plantarum 24, Isolated From the Marula Fruit (Sclerocarya birrea), has Probiotic Properties and Harbors Genes Encoding the Production of Three Bacteriocins

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Abstract

Lactobacillus plantarum 24, isolated from marula fruit grows at pH 4.0 and tolerates acid levels and bile concentrations normally present in the human gastro-intestinal tract. Wistar rats that have been administered L. plantarum 24 showed no signs of discomfort or abnormal behavior. Tissue samples from the liver, spleen and intestine appeared normal. Furthermore, strain 24 harbors the genes encoding plantaricins A, F, and NC8α, a gene encoding immunity to plantaricin, and an ABC transporter similar in sequence to that reported for plantaricin G. At least one antimicrobial peptide within the size range of plantaricins A, F, and NC8α has been detected on a tricine-SDS–PAGE gel. Little is known about the microbial population in marula. This is the first report of a L. plantarum strain from marula fruit with bacteriocin genes and probiotic properties.

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Acknowledgment

The National Research Foundation (NRF), South Africa, for funding.

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Authors and Affiliations

  1. Department of Microbiology, Stellenbosch University, Stellenbosch, 7600, South Africa

    Willem A. Prins, Marlie Botha, Marelize Botes, Michele de Kwaadsteniet, Akihito Endo & Leon M. T. Dicks

  2. Private Bag X1, Matieland, Stellenbosch, 7602, South Africa

    Leon M. T. Dicks

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  1. Willem A. Prins
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  2. Marlie Botha
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  3. Marelize Botes
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  4. Michele de Kwaadsteniet
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  6. Leon M. T. Dicks
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Correspondence to Leon M. T. Dicks.

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Prins, W.A., Botha, M., Botes, M. et al. Lactobacillus plantarum 24, Isolated From the Marula Fruit (Sclerocarya birrea), has Probiotic Properties and Harbors Genes Encoding the Production of Three Bacteriocins. Curr Microbiol 61, 584–589 (2010). https://doi.org/10.1007/s00284-010-9656-8

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