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Molecular Relationships Between Saccharomyces cerevisiae Strains Involved in Winemaking from Mendoza, Argentina

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Abstract

Three molecular typing techniques were applied to assess the molecular relationships of Saccharomyces cerevisiae strains isolated from winery equipment, grapes, and spontaneous fermentation in a cellar located in “Zona Alta del Río Mendoza” (Argentina). In addition, commercial Saccharomyces strains widely used in this region were also included. Interdelta PCR typing, mtDNA restriction analysis, and microsatellite (SSR) genotyping were applied. Dendrograms were constructed based on similarity among different patterns of bands. The combination of the three techniques discriminated 34 strains among the 35 isolates. The results of this study show the complex relationships found at molecular level among the isolates that share the same ecological environment, i.e., the winemaking process. With a few exceptions, the yeast isolates were generally clustered in different ways, depending on the typing technique employed. Three clusters were conserved independently of the molecular method applied. These groups of yeasts always clustered together and had high degree of similarity. Furthermore, the dendrograms mostly showed clusters combining strains from winery and fermentation simultaneously. Most of the commercial strains included in this study were clustered separately from the other isolates analyzed, and just a few of them grouped with the strains mainly isolated from spontaneous fermentation. Only one commercial strain was clustered repetitively with a noncommercial strain isolated from spontaneous fermentation in the three dendrograms. On the other hand, this study has demonstrated the importance of selecting an appropriate molecular method according to the main objectives of the research.

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Acknowledgments

This study was supported by Viticulture Regional Project MZASJ O7 from the Instituto Nacional de Tecnología Agropecuaria (INTA) (and Project PDT 32/06 from Dinacyt, Uruguay). L.M. is a fellow of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Programa de Doctorado en Ciencias Biológicas (PROBIOL), Universidad Nacional de Cuyo.

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Authors and Affiliations

  1. Instituto Nacional de Tecnología Agropecuaria (INTA), EEA Mendoza and EEA La Consulta, San Martín 3853, 5507, Lujan de Cuyo, Mendoza, Argentina

    Laura Mercado, Ricardo W. Masuelli & Mariana Combina

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

    Ricardo W. Masuelli & Mariana Combina

  3. Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina

    Laura Mercado & Ricardo W. Masuelli

  4. Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Montevideo, Uruguay

    Sandra Jubany & Carina Gaggero

Authors
  1. Laura Mercado
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  2. Sandra Jubany
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  3. Carina Gaggero
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  4. Ricardo W. Masuelli
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  5. Mariana Combina
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Correspondence to Mariana Combina.

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Mercado, L., Jubany, S., Gaggero, C. et al. Molecular Relationships Between Saccharomyces cerevisiae Strains Involved in Winemaking from Mendoza, Argentina. Curr Microbiol 61, 506–514 (2010). https://doi.org/10.1007/s00284-010-9645-y

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  • DOI: https://doi.org/10.1007/s00284-010-9645-y

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