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Tightly Regulated Expression Vectors for Ochrobactrum anthropi

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Abstract

Genetic studies of Ochrobactrum anthropi, a bacterial species important in bioremediation and biopesticide degradation, are hindered by the lack of suitably regulated gene expression system. A tightly regulated gene-expression system was developed for O. anthropi using the lacIq gene and a re-engineered coliphage T5 promoter containing completely symmetrical DNA segment that binds more efficiently to the lactose repressor. The β-galactosidase activity was increased 57-fold when the expression of the re-engineered T5 promoter was induced. The degree of induction was controllable by varying the concentration of inducer isopropyl-β-d-thiogalactopyranoside.

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Authors and Affiliations

  1. Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 1410 Prices Fork Rd, Blacksburg, VA, 24061, USA

    Hamzeh Alqublan, Mohamed N. Seleem, Stephen M. Boyle & Nammalwar Sriranganathan

  2. Institute for Critical Technology and Applied Science, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA

    Mohamed N. Seleem

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  1. Hamzeh Alqublan
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  2. Mohamed N. Seleem
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  3. Stephen M. Boyle
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  4. Nammalwar Sriranganathan
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Correspondence to Nammalwar Sriranganathan.

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Alqublan, H., Seleem, M.N., Boyle, S.M. et al. Tightly Regulated Expression Vectors for Ochrobactrum anthropi . Curr Microbiol 60, 242–247 (2010). https://doi.org/10.1007/s00284-009-9532-6

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  • DOI: https://doi.org/10.1007/s00284-009-9532-6

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