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Genetic Diversity of Plant Growth Promoting Rhizobacteria Isolated from Rhizospheric Soil of Wheat Under Saline Condition

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Abstract

In this study, a total of 130 rhizobacteria was isolated from a saline infested zone of wheat rhizosphere, and screened for plant growth promoting (PGP) traits at higher salt (NaCl) concentrations (2, 4, 6, and 8%). The results revealed that 24 rhizobacterial isolates were tolerant at 8% NaCl. Although all the 24 salt tolerable isolates produced indole-3-acetic acid (IAA), while 10 isolates solubilized phosphorus, eight produced siderophore, and six produced gibberellin. However, only three isolates showed the production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase. Diversity was analyzed through 16S rDNA-RFLP, and of these isolates with three tetra cutter restriction enzymes (HaeIII, AluI, and MspI), the representative cluster groups were identified by 16S rDNA sequencing. Bacillus and Bacillus-derived genera were dominant which showed PGP attributes at 8% NaCl concentration. Out of 24 isolates, nitrogen fixing ability (nif H gene) was detected in the two isolates, SU18 (Arthrobacter sp.) and SU48.

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Acknowledgments

This research work was supported by the Department of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow, India and ICAR-Network Project, Indian Council of Agricultural Research, Government of India, New Delhi. Authors are thankful to the funding agencies.

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Authors and Affiliations

  1. Department of Environmental Sciences, B.B. Ambedkar University, Vidya Vihar, Rai Bareilly Road, Lucknow, Uttar Pradesh, 226 025, India

    Sudhir K. Upadhyay & Devendra P. Singh

  2. Biotechnology Division, North-East Institute of Science and Technology (CSIR), Jorhat, 785006, Assam, India

    Ratul Saikia

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  1. Sudhir K. Upadhyay
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  2. Devendra P. Singh
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Correspondence to Sudhir K. Upadhyay.

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Upadhyay, S.K., Singh, D.P. & Saikia, R. Genetic Diversity of Plant Growth Promoting Rhizobacteria Isolated from Rhizospheric Soil of Wheat Under Saline Condition. Curr Microbiol 59, 489–496 (2009). https://doi.org/10.1007/s00284-009-9464-1

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  • DOI: https://doi.org/10.1007/s00284-009-9464-1

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