Abstract
A cellulase-producing bacterium strain was isolated from soil that produced novel thermoalkalotolerant cellulases after growth on CMC-Na agar screening plate at 37°C. It was identified as Escherichia coli using the method of 16S rRNA and intergenic spacer gene analysis combined with morphological, physiological, and biochemical tests. Three major components of the cellulases [carboxymethyl cellulase (CMCase), filter paper cellulase, and β-glucosidase] were produced with maximal activities (0.23, 0.08, and 0.15 U/ml) and maximum specific activities 4.13, 0.56, and 0.50 U/mg protein after 72, 96, and 120 h growth, respectively. Maximum CMCase activity was measured at 50°C and pH 6.0, respectively, and it also retained more than 60% of its maximal activity for at least 20 min at 50–70°C and 10 min at 80°C, respectively, and retained approximately 50% of its maximal activity after incubating at 90°C for 10 min. The enzyme could be applied in bioconversion of lignocellulosic agricultural wastes.
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Acknowledgments
This study was supported by a key project of Zhejiang Government No. 2008C24011 and by the Hi-Tech Research and Development Program of China (Nos. 2008AA10Z132 and 2006AA10A119).
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Li, Xh., Bhaskar, R., Yang, Hj. et al. Screening and Identification of New Isolate: Thermostable Escherichia coli with Novel Thermoalkalotolerant Cellulases. Curr Microbiol 59, 393–399 (2009). https://doi.org/10.1007/s00284-009-9450-7
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DOI: https://doi.org/10.1007/s00284-009-9450-7