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Simple Method to Accurately Differentiate Candida albicans Isolates Concurrently Using Polymorphic Patterns of PCR-Amplified, Species-Specific Nuclear and Mitochondrial Targets

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Abstract

We describe a simple method to accurately differentiate Candida albicans isolates by concurrent use of the restriction enzyme digestion patterns for PCR products, targeting two species-specific DNA regions originating from genetically different sources, the nuclear and mitochondrial genomes. The target sequence we used as the nuclear gene was derived from the PHO85 gene, a negative regulator of the PHO system, in which we found a restriction size polymorphism within the two alleles of PHO85 in the diploid genome of this fungus. The mitochondrial target was derived from EO3, a species-specific DNA fragment possessing a small size polymorphism among various clinical isolates. Our results should provide a new tool for molecular epidemiological surveys of patients suffering from candidiasis caused by C. albicans.

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Acknowledgments

We are grateful to the National BioResource Project (http://www.nbrp.jp/) for the pathogenic microbes (Medical Mycology Research Center, Chiba University, Japan) and to Meiji Pharmaceutical University (Dr. T. Sugita, Tokyo) for provision of C. albicans isolates. We are also indebted to Emeritus Professor Y. Fukazawa for his invaluable cooperation and encouragement throughout the present study.

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Authors and Affiliations

  1. Division of Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takada, Kofu, Yamanashi, 400-8511, Japan

    Yozo Miyakawa & Takuya Ozawa

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  1. Yozo Miyakawa
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  2. Takuya Ozawa
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Correspondence to Yozo Miyakawa.

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Miyakawa, Y., Ozawa, T. Simple Method to Accurately Differentiate Candida albicans Isolates Concurrently Using Polymorphic Patterns of PCR-Amplified, Species-Specific Nuclear and Mitochondrial Targets. Curr Microbiol 58, 164–169 (2009). https://doi.org/10.1007/s00284-008-9300-z

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  • DOI: https://doi.org/10.1007/s00284-008-9300-z

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