Abstract
The only quorum sensing (QS) system shared by Gram-positive and Gram-negative bacteria involves the production of autoinducer-2 (AI-2). However, there have been no reports concerning the AI-2 in Streptococcus suis serotype 2 (SS2). In this study, we investigated AI-2 production and analyzed the relationship between the transcription level of luxS and pfs and AI-2 production. A homologue of luxS, the gene required for AI-2 synthesis in Vibrio harveyi, was isolated from the SS2 genome. V. harveyi BB170 bioassay demonstrated luxS functionality in SS2 and its ability to complement the luxS-negative phenotype of Escherichia coli DH5α. Further studies showed that AI-2 activity peaked in the late exponential phase, and sodium chloride or glucose increased the production of AI-2. Real-time PCR showed that the level of transcription of pfs is highly correlated with the level of AI-2 production, while the level of transcription of luxS does not correlate with the level of AI-2 production. The results presented here demonstrate that SS2 can secrete AI-2 and that the profile of pfs transcription is highly correlated with the level of AI-2 production.
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Acknowledgment
This work was supported by funds from the Chinese National Programs for Fundamental Research and Development (2006CB504403).
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Han, X.G., Lu, C.P. Detection of Autoinducer-2 and Analysis of the Profile of luxS and pfs Transcription in Streptococcus suis Serotype 2. Curr Microbiol 58, 146–152 (2009). https://doi.org/10.1007/s00284-008-9291-9
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DOI: https://doi.org/10.1007/s00284-008-9291-9