Abstract
A cryptic plasmid from Lactobacillus plantarum LR1, designated pLR1, was sequenced and characterized. It consisted of a 2066-bp circular molecule with a G + C content of 52.7%. The plasmid pLR1 was predicted to contain five putative ORFs, in which ORF1 shared 93% and 92% identity with Rep proteins of pLP1 and pC30il, members of rolling-circle replication (RCR) pC194 family. Detection of single-stranded DNA (ssDNA) intermediates by Southern hybridization and mung bean nuclease treatment confirmed that pLR1 replicated via the RCR mechanism. Accumulation of ssDNA in rifampicin-treated strains implied that the host-coded RNA polymerase was involved in the conversion of ssDNA to double-stranded DNA (dsDNA). Furthermore, the copy number of pLR1 was estimated to be 36 in each cell by real-time polymerase chain reaction.
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Acknowledgments
This work was supported by National High-Tech R&D Program Grants (2006AA10Z317, 2007AA10Z354, and 2006BAB04A06) from the Ministry of Science and Technology of the People’s Republic of China.
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Li, R., Zhai, Z., Yin, S. et al. Characterization of a Rolling-Circle Replication Plasmid pLR1 from Lactobacillus plantarum LR1. Curr Microbiol 58, 106–110 (2009). https://doi.org/10.1007/s00284-008-9280-z
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DOI: https://doi.org/10.1007/s00284-008-9280-z