Skip to main content
SpringerLink
Log in

Inhibition of Vancomycin and High-Level Aminoglycoside-Resistant Enterococci Strains and Listeria monocytogenes by Bacteriocin-Like Substance Produced by Enterococcus faecium E86

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Three hundred and thirty nine lactic bacteria strains isolated from food samples were screened for antimicrobial activity. Only one strain isolated from meat pie and identified as Enterococcus faecium produced a bacteriocin-like inhibitory substance (BLIS) showing activity against Enterococcus, Leuconostoc, Lactobacillus, Listeria, Corynebacterium and Staphylococcus aureus. The BLIS produced was resistant to acid and alkali treatment and 121ºC for 15 min. The addition of BLIS in BHI contaminated with Listeria monocytogenes decreased the contamination in 4.8 log cycles in 24 h. The inhibition of listeria was also obtained in milk. Forty multiresistant enterococci strains were inhibited in the well-diffusion test. Two vancomycin resistant strains tested in liquid with BLIS were also inhibited. The BLIS producer showed no pathogenicity marker.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Aymerich T, Holo H, Havarstein LS et al (1996) Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins. Appl Environ Microbiol 62:1676–1682

    PubMed  CAS  Google Scholar 

  2. Bhunia AK, Johnson MC, Ray B (1987) Direct detection of an antimicrobial peptide of Pediococcus acidilacticii in sodium dodecil sulfate–polyacrylamide gel eletrophoresis. J Ind Microbiol 2:319–322

    Article  CAS  Google Scholar 

  3. Chow JW, Thal LA, Perri MB et al (1993) Plasmid-associated hemolysin and aggregation substance production contribute to virulence in experimental enterococcal endocarditis. Antimicrob Agents Chemoth 37:2474–2477

    CAS  Google Scholar 

  4. Cintas LM, Casaus P, Havarstein LS et al (1998) Enterocin L50A and L50B, two novel bacteriocins from Enterococcus faecium L50, are related staphylococcal hemolysis. J Bacteriol 180:1988–1994

    PubMed  CAS  Google Scholar 

  5. Clinical Laboratory Standard Institute (2005) Performance standards for antimicrobial susceptibility testing, approved standard M100–S15. Clinical Laboratory Standard Institute, Wayne, PA

    Google Scholar 

  6. Del Campo R, Tenorio C, Jiménez-Díaz R. et al. (2001). Bacteriocin production in vancomycin-resistant and vancomycin-susceptible Enterococcus Isolates of different origins. Antimicrob Agents Chemoth 45:3 905–912

    Google Scholar 

  7. Gálvez A, Valdivia E, Abriouel H et al (1998) Isolation and characterization of enterocin EJ97, a bacteriocin produced by Enterococcus faecalis EJ97. Arch Microbiol 171:59–65

    Article  PubMed  Google Scholar 

  8. Gibbs PA (1987) Novel uses for lactic acid fermentation in food preservation. J Appl Bacteriol 63:51–58

    Google Scholar 

  9. Giraffa G (2003) Functionality of enterococci in dairy products. Int J. Food Microbiol 88:215–222

    Article  PubMed  CAS  Google Scholar 

  10. Héchard Y, Sahl H (2002) Mode of action of modified and unmodified bacteriocins from Gram-positive bacteria. Biochimie 84:545–557

    Article  PubMed  Google Scholar 

  11. Hugas M, Garriga M, Aymerich MT (2003) Functionality of enterococci in meat products. Int J Food Microbiol 88:223–233

    Article  PubMed  CAS  Google Scholar 

  12. Kayser FH (2003) Safety aspects of enterococci from the medical point of view. Int J Food Microbiol 88:255–262

    Article  PubMed  CAS  Google Scholar 

  13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  14. Libertin CR, Dumitru R, Stein DS (1992) The hemolysin/bactreriocin produced by enterococci is a marker of pathogenicity. Diagn Microbiol Infect Dis 15:115–120

    Article  PubMed  CAS  Google Scholar 

  15. Lukasová J, Sustacková A (2003) Enterococci and antibiotic resistance. Acta Vet Brno 72:315–323

    Google Scholar 

  16. Merquior VL, Peralta JM, Facklam R et al (1994) Analysis of electrophoretic whole-cell protein profiles as a tool for characterization of Enterococcus species. Curr Microbiol 28:149–153

    Article  CAS  Google Scholar 

  17. Murray BE (2000) Vancomycin-resistant enterococcal infections. New Engl J Med 342:710–721

    Article  PubMed  CAS  Google Scholar 

  18. Oliveira SS, Nascimento JS, Póvoa DC et al (1998) Genetic analysis of the bacteriocin-encoding plasmids pRJ6 and pRJ9 of Staphylococcus aureus using transposons as insertional mutagens and cloning of genes involved in bacteriocin production. J Appl Microbiol 85:972–984

    PubMed  Google Scholar 

  19. Sabia C, Niederhausern S, Guerrieri E et al (2008) Detection of bacteriocin production and virulence traits in vancomycin-resistant enterococci of different sources. J Appl Microbiol 108:970–979

    Article  Google Scholar 

  20. Schillinger U, Lücke FK (1987) Identification of lactobacilli from meat and products isolated from meat. Food Microbiol 4:199–208

    Article  Google Scholar 

  21. Schillinger V, Lucke FK (1989) Antimicrobial activity of Lactobacillus sake isolated from meat. Appl Environ Microbiol 39:189–195

    Google Scholar 

  22. Weaver KE, Clewell D (1988) Regulation of the pAD1 sex pheromone response in Enterococcus faecalis: construction and characterization of lacZ transcriptional fusions in a key control region of the plasmid. J Bacteriol 170:4343–4352

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Ilha do Fundão, Rio de Janeiro, RJ, Brazil

    Marco Antônio Lemos Miguel & Ângela Cristina Dias de Castro

  2. Instituto de Microbiologia Prof. Paulo de Góes, Lab. Microbiologia de Alimentos, Rio de Janeiro, RJ, Brazil

    Marco Antônio Lemos Miguel & Ângela Cristina Dias de Castro

  3. Escola de Química, Universidade Federal do Rio de Janeiro, CT, Bloco E, CEP 21941-900, Rio de Janeiro, RJ, Brazil

    Selma Ferreira Gomes Leite

Authors
  1. Marco Antônio Lemos Miguel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ângela Cristina Dias de Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Selma Ferreira Gomes Leite
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marco Antônio Lemos Miguel.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lemos Miguel, M.A., Dias de Castro, Â.C. & Ferreira Gomes Leite, S. Inhibition of Vancomycin and High-Level Aminoglycoside-Resistant Enterococci Strains and Listeria monocytogenes by Bacteriocin-Like Substance Produced by Enterococcus faecium E86. Curr Microbiol 57, 429–436 (2008). https://doi.org/10.1007/s00284-008-9224-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-008-9224-7

Keywords

Search

Navigation