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Isolation and Identification of an Endophytic Strain EJS-3 Producing Novel Fibrinolytic Enzymes

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Abstract

An endophytic strain EJS-3, which produces a novel fibrinolytic enzyme, was screened from root tissue of Stemona japonica (Blume) Miq, a chinese traditional medicine. This strain was identified as Paenibacillus polymyxa (DQ120522) by morphological, physiological, and biochemical tests and 16S rRNA gene sequence analysis. Two serine-type fibrinolytic enzymes with a relative molecular weight about 118 and 49 kDa, respectively, which are larger than known fibrinolytic enzymes, were found by the SDS–fibrin zymogram or by fibrin-inhibitor zymography gels. No work on P. polymyxa-producing fibrinolytic enzymes has been reported.

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Authors and Affiliations

  1. College of Food Science and Technology, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China

    Fengxia Lu, Lijun Sun, Zhaoxin Lu, Xiaomei Bie, Yaowei Fang & Shu Liu

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  1. Fengxia Lu
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  2. Lijun Sun
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  3. Zhaoxin Lu
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Correspondence to Zhaoxin Lu.

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Lu, F., Sun, L., Lu, Z. et al. Isolation and Identification of an Endophytic Strain EJS-3 Producing Novel Fibrinolytic Enzymes. Curr Microbiol 54, 435–439 (2007). https://doi.org/10.1007/s00284-006-0591-7

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  • DOI: https://doi.org/10.1007/s00284-006-0591-7

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