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Verification and Rapid Identification of Soybean Rhizobia in Indian Soils

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Abstract

Sixty root nodule isolates of soybean rhizobia indigenous to eight field sites in India were characterized using PCR-RFLP for repeated sequence RSα a 1195-bp DNA fragment, indole acetic acid production, and nitrogenase activity. Site-dependent variations were observed in terms of IAA production and nitrogenase activities. RSα was conserved in slow-growing soybean rhizobia across locations and sites and was absent in other Rhizobiaceae members and other bacterial genera. The results suggest that RSα can be a useful molecular marker for slow-growing soybean rhizobia. The study also showed the low presence of soybean nodulating fast growers in Indian soils.

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Acknowledgments

Thanks go to the National Phytotron Facility, IARI, for conducting the nodulation tests. B.N is indebted to IARI, New Delhi, for a junior research fellowship

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  1. Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    K. Annapurna, N. Balakrishnan & L. Vital

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  1. K. Annapurna
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  2. N. Balakrishnan
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  3. L. Vital
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Correspondence to K. Annapurna.

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Annapurna, K., Balakrishnan, N. & Vital, L. Verification and Rapid Identification of Soybean Rhizobia in Indian Soils. Curr Microbiol 54, 287–291 (2007). https://doi.org/10.1007/s00284-006-0423-9

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  • DOI: https://doi.org/10.1007/s00284-006-0423-9

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