Abstract
The immunodominant antigen A, IsaA, of Staphylococcus aureus was found to include a putative soluble lytic transglycosylase domain in its C-terminal region. Since the presence of this distinctive domain suggested that the protein might participate in peptidoglycan turnover, as indicated in Gram-negative bacteria, its cellular location was investigated. The protein was found not only in the culture supernatant but also in the cell wall fraction. To estimate its physiological role for the bacterium, its cell surface distribution was studied by immunoelectron microscopy. Protein A-gold particles binding to the immune complex were mainly located on the septal region of the bacterial cell surface. These data suggested that IsaA might be involved in bacterial cell separation through a preferential interaction with peptidoglycan chain.
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Acknowledgments
We are grateful to Dr. S. Yamada, Kawasaki Medical College, for S. aureus 2PF-18; Dr. S. Sakurai, Kohno Clinical Medicine Research Institute, for the N-terminal analysis; and Ms. C. Sasaki, Institute for Ultrastructural Morphology, Postgraduate, St. Marianna University School of Medicine, for technical assistance. We also thank Dr. I. Kondo, Jikei University School of Medicine, and Dr. T. Wadström, Lund University, for critical suggestions.
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Sakata, N., Terakubo, S. & Mukai, T. Subcellular Location of the Soluble Lytic Transglycosylase Homologue in Staphylococcus aureus. Curr Microbiol 50, 47–51 (2005). https://doi.org/10.1007/s00284-004-4381-9
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DOI: https://doi.org/10.1007/s00284-004-4381-9