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Sequencing and Characterization of Plasmid pUIBI-1 from Bacillus thuringiensis Serovar entomocidus LBIT-113

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Abstract

Plasmid pUIBI-1 from Bacillus thuringiensis svr. entomocidus was sequenced and its replication mechanism analyzed. Sequence analysis revealed that pUIBI-1 contains 4671 bp and a 32% GC content. Plasmid pUIBI-1 also includes at least seven putative open reading frames (ORFs) encoding for proteins ranging from 5 to 50 kDa. ORF-1 encodes for a putative 16-kDa Rep protein, which lacks homology with proteins of similar function. ORF2 encodes for a protein of 50 kDa and shows homology with Mob proteins of plasmids pLUB1000 from Lactobacillus hilgardii (32.2%) and pGI2 from B. thuringiensis (33.7%). Detection of single-stranded DNA (ssDNA) intermediates indicated that pUIBI-1 replicates by the rolling-circle replication mechanism, as demonstrated by S1 treatment and Southern hybridization under non-denaturing conditions.

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Authors and Affiliations

  1. Centro Multidisciplinario de Estudios en Biotecnología-FMVZ, Univ. Michoacana de San Nicolás de Hidalgo, Apdo. Postal 53, Administración Chapultepec, 58262 Morelia, Michoacán, Mexico

    Joel E. López-Meza

  2. Instituto de Ciencias Agricolas, Universidad de Guanajuato, Apdo. Postal 311, 36500 Irapuato, Gto., Mexico

    J. Eleazar Barboza-Corona

  3. Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del I.P.N., Apdo. Postal 629, 36500 Irapuato, Gto., Mexico

    M. Cristina Del Rincón-Castro &  Jorge E. Ibarra

Authors
  1. Joel E. López-Meza
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  2. J. Eleazar Barboza-Corona
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  3. M. Cristina Del Rincón-Castro
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  4. Jorge E. Ibarra
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López-Meza, ., Barboza-Corona, ., Del Rincón-Castro, . et al. Sequencing and Characterization of Plasmid pUIBI-1 from Bacillus thuringiensis Serovar entomocidus LBIT-113. Curr Microbiol 47, 395–399 (2003). https://doi.org/10.1007/s00284-003-4041-5

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  • DOI: https://doi.org/10.1007/s00284-003-4041-5

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