Abstract
The immune system is governed by dynamic events involving in part direct intercellular interactions between an immune cell and other cells or the cell’s environment. Owing to its unique optical characteristics, the eye offers remarkable opportunities for the analysis of the immune system by intravital microscopy. In this review, we present a brief overview of the current state of knowledge of leukocyte trafficking in each of three anatomically distinct and medically important regions of the eye (cornea, iris, retina) as determined by the application of intravital microscopy to animal models of disease. Additionally, we discuss the use of ocular imaging in patients and volunteers. Finally, we examine the future prospects for this field in terms of its potential for impacting our understanding of fundamental immunological phenomena.
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Acknowledgments
We would like to acknowledge the support of National Institute of Health grants EY015448 (E.J.L), EY006484 and EY013093 (J.T.R., D.B.S, T.K.), as well as Research to Prevent Blindness awards to JTR and the CEI. We are also grateful for support from The Stan and Madelle Rosenfeld Family Trust, the Bauman Foundation, and OHSU Foundation. We would also like to acknowledge the valuable input of Steve Planck and the technical assistance of Mischa Ronick, Shannon Douglas, and Kellen Galster as well as Lyndell Lim for constructive comments on the manuscript.
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Supplementary video 1 Representative whole-mount confocal image of an inflamed iris prepared at 24 h post OVA + LPS challenge (see Fig. 2). OVA-specific T cells are stained with CMTMR (red, arrow A), probable APCs are labeled by uptake of Alexa Green 488-conjugated ovalbumin (arrow B), and blood vessels are identifiable by labeling with Texas Red Tomato lectin (arrow C), as described previously [66]. This image illustrates the physical association of antigen-specific T cells with APCs.(MPG 511 KB).
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Spencer, D.B., Lee, E.J., Kawaguchi, T. et al. In vivo imaging of the immune response in the eye. Semin Immunopathol 30, 179–190 (2008). https://doi.org/10.1007/s00281-008-0107-6
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DOI: https://doi.org/10.1007/s00281-008-0107-6