Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E₂, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients

Abstract.

We analyzed the ability of the bone marrow (BM) stromal cells to achieve confluence and their proliferative capacity in BM primary cultures from 30 untreated lung cancer patients (LCP), 27 breast cancer patients (BCP), and 30 normal controls (NC) when these confluent cells were induced to proliferate following four continuous subcultures. Moreover, we evaluated the production of interleukin-1 beta (IL-1β), transforming growth factor beta 1 (TGF-β1), fibronectin, and prostaglandin E₂ (PGE₂) by pure fibroblasts (fourth passage). A fibroblast colony-forming units (CFU-F) assay was used to investigate the proliferative and confluence capacity. Levels of IL-1β, TGF-β1, and fibronectin in conditioned mediums (CM) of fibroblast cultures were measured by enzyme-linked immunosorbent assay (ELISA) kit and PGE₂ by radioimmunoassay (RIA) kit. Confluence was achieved in the 60% of LCP and 78% of BCP primary cultures compared with 100% of NC, and only fibroblasts from seven LCP and six BCP cultures had the capacity to proliferate following four subcultures. Levels of IL-1β were below 10 pg/ml in both patient groups, while NC had a mean value of 5882.57±221.61 pg/ml. Levels of TGF-β1 in BCP were lower than NC values (P<0.05). LCP and BCP had significantly decreased levels of fibronectin when compared to NC values (P<0.05 and P<0.01, respectively). Levels of PGE₂ in LCP were higher compared to NC (P<0.01). In conclusion, BM fibroblasts from LCP and BCP presented a defective proliferative and confluence capacity, and this deficiency may be associated with the alteration of IL-1β, TGF-β1, fibronectin, and PGE₂ production.