Abstract
Stating backround
This study was designed to examine the entire intramuscular nerve distribution pattern of various human skeletal muscles in fetuses.
Methods
In the present study rhomboid major, trapezius, long head of the biceps femoris and masseter muscles were investigated in five 18 weeks old fetal cadavers. Anatomical microdissection was applied to one fetal cadaver. In two fetuses, the extramuscular (main), major and minor nerve branches, and anastomosis were examined using Sihler’s staining and labeling. In the remaining two fetuses, consecutive slices with 0.5 mm interval and 5 μm thickness were obtained from each skeletal muscle. These slices were stained with S100 for the demonstration of the nerve fibers and thereafter 3D reconstruction images were constituted using PC software.
Results
Anatomical microdissection, Sihler’s staining and computerized reconstruction methods were compared to demonstrate the intramuscular nerve distribution pattern. Demonstration of the intramuscular minor nerve branches and anastomosis showed difficulties in anatomical dissected specimens when compared with three-dimensionally reconstructed images and specimens obtained with Sihler’s staining technique. Nevertheless, anatomical dissection is a simple method whereas Sihler’s technique and computer aided 3D reconstruction are complex methods and take a long time to complete.
Conclusion
The obtained information exposed that staining technique and the 3D reconstructions appeared to provide better results than did anatomical dissection.
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References
Boenish T, Farmilo AJ, Stead RH, Key M, Welcher R, Harvey R, Atwood KN (2001) Handbook immunochemical staining methods, 3rd edn. DakoCytomation, Carpinteria, pp 5–47
Born G (1883) Die Plattenmodelliermethode. Archiv f Mikroskop Anatomie 22:584–599
Botha GSM (1957) The anatomy of phrenic nerve termination and the motor innervation of the diaphragm. Thorax 12:50–56
Glaser EM, Van der Loos H (1965) A semi-automatic computer-microscope for the analysis of neuronal morphology. IEEE Trans Biomed Eng 12:22–31
Gülekon N, Peker T, Liman F, Anıl A, Turgut H.B (2002) Demonstration of the nerve distribution of the extraocular muscles in rabbits (Oryctolagus cuniculus). Cur Eye Res 25:179–188
Heimer L, Robards MJ (1981) Neuroanatomical tract-tracing methods. Plenum, New York, pp 43–53
His W (1887) Ueber die Methoden der plastischen Rekonstruktion und über deren Bedeutung für Anatomie und Entwicklungsgeschichte. Anat Anz 2:382–392
Homma T, Sakai T (1991) Ramification pattern of intermetacarpal branches of the deep branch (ramus profundus) of the ulnar nerve in the human hand. Acta Anat 141:139–144
Homma T, Sakai T (1992) Thenar and hypothenar muscles and their innervation by the ulnar nerve and median nerves in the human hand. Acta Anat 145:44–49
Liem RSB, VanWilligen JD (1988) In toto staining and preservation of peripheral nervous tissue. Stain Technol 63:113–120
Liu J, Kumar VP, Lau HK, Pereira BP, Shen Y, Pho RWH (1997) Neuromuscular compartments in the long head of triceps: a morphological study in rabbits. Muscle Nerve 20:897–899
Liu J, Kumar VP, Shen Y, Lau HK, Pereira BP, Pho RWH (1997) Modified Sihler’s technique for studying the distribution of intramuscular nerve branches in mammalian skeletal muscle. Anat Rec 247:137–144
Moody D, Lozanoff S (1999) Surf driver: rapid and reliable 3D reconstruction, Surf Driver 3.5 Manual
Mu L, Sanders I (1998) Neuromuscular specializations of the pharyngeal dilator muscles: I. Compartments of the canine geniohyoid muscle. Anat Rec 250:146–153
Mu L, Sanders I (1999) Neuromuscular organization of the canine tongue. Anat Rec 256:412–424
Mu L, Sanders I (2000) Sensory nerve supply of the human oro- and laryngopharynx: a preliminary study. Anat Rec 258:406–420
Parratte B, Tatu L, Vuillier F, Diop M, Monnier G (2002) Intramuscular distribution of nerves in the human triceps surae muscle: anatomical bases for treatment of spastic drop foot with botulinum toxin. Surg Radiol Anat 24:91–96
Peker T, Turgut HB, Gülekon N, Anıl A (2001) Demonstration of the nerve distribution of the masticatory muscles in rabbits (Oryctolagus cuniculus). Anat Histol Embryol 30:225–229
Peker T, Gülekon N, Anıl A, Turgut HB, Liman F (2003) Clearing and embedding in polyester resin for demonstrating the nerve distribution pattern of skeletal muscles. Biotech Histochem 78:187–190
Peker T, Gülekon N, Anıl A, Turgut HB, Karaköse M (2006) Observation of therelationship between the shape of skeletal muscle and their nerve distribution pattern: a transparent and microanatomic study. Plast Reconstr Surg 117:165–176
Sihler C (1895) Ueber Muskelspindeln und intramuskulare Nervendigungen bei Schlangen und Fröschen. Arch Mikros Anat Entwickl 46:709–723
Wu BL, Sanders I (1992) A technique for demonstrating the nerve supply of whole larynges. Arc Otolaryngol Head Neck Surg 118:822–827
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Gülekon, N., Peker, T., Turgut, H.B. et al. Qualitative comparison of anatomical microdissection, Sihler’s staining and computerized reconstruction methods for visualizing intramuscular nerve branches. Surg Radiol Anat 29, 373–378 (2007). https://doi.org/10.1007/s00276-007-0225-1
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DOI: https://doi.org/10.1007/s00276-007-0225-1