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In Vitro Comparison of the Antiproliferative Effects of Rhenium-186 and Rhenium-188 on Human Aortic Endothelial Cells

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Abstract

Purpose

Rhenium-186 (186Re) and rhenium-188 (188Re) are promising radionuclides for the inhibition of restenosis after percutaneous transluminal angioplasty or other vascular interventions. Until now the maximal dose tolerance of endothelial cells has not been clearly known.

Materials and Methods

To characterize the effects of local irradiation treatment, human aortic endothelial cells (ECs) were incubated with different doses of 186Re and 188Re. Two days after plating, ECs received treatment for a period of 5 days. The total radiation doses applied were 1, 4, 8, 16, and 32 Gy. On days 1, 3, 5, 7, and 12 after initial rhenium incubation, cell growth, clonogenic activity, cell-cycle distribution, and cytoskeletal architecture were evaluated.

Results

From the first day on, a dose-dependent growth inhibition was observed. Cumulative doses of ≥32 Gy caused a weak colony formation and significant alterations in the cytoskeletal architecture. An increased fraction of cells in G2/M phase was seen for cumulative radiation doses of ≥16 Gy. Interestingly, there were no significant differences between 186Re and 188Re.

Conclusion

Even for low dose rates of β particles a dose-dependent proliferation inhibition of ECs is seen. Doses beyond 32 Gy alter the cytoskeletal architecture with possibly endothelial dysfunction and late thrombosis.

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Authors and Affiliations

  1. Department of Diagnostic and Interventional Radiology, University Hospital of Tuebingen, Hoppe-Seyler-Str. 3, 72076, Tuebingen, Germany

    Alexander Sauter, Daniel Arthasana, Benjamin Wiesinger, Joerg Schmehl, Klaus Brechtel, Rüdiger Bantleon, Claus Claussen & Rainer Kehlbach

  2. Department of Nuclear Medicine, University Hospital of Tuebingen, Otfried-Mueller-Str. 14, 72076, Tuebingen, Germany

    Helmut Dittmann & Maren Pritzkow

Authors
  1. Alexander Sauter
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  2. Daniel Arthasana
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  3. Helmut Dittmann
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  5. Benjamin Wiesinger
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  10. Rainer Kehlbach
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Correspondence to Alexander Sauter.

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Sauter, A., Arthasana, D., Dittmann, H. et al. In Vitro Comparison of the Antiproliferative Effects of Rhenium-186 and Rhenium-188 on Human Aortic Endothelial Cells. Cardiovasc Intervent Radiol 34, 816–823 (2011). https://doi.org/10.1007/s00270-010-0003-z

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  • DOI: https://doi.org/10.1007/s00270-010-0003-z

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