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Effects of Time on Ultrastructural Integrity of Parathyroid Tissue Before Cryopreservation

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Abstract

Background

Cryopreservation of parathyroid tissue is used in the surgical treatment of secondary hyperparathyroidism. After surgical resection, the tissue is temporarily maintained in a cell culture solution until it arrives at the specialized laboratory where the cryopreservation process will take place. The present study evaluates the time that the human hyperplastic parathyroid gland tissue can wait before cryopreservation, based on parathyroid cell ultrastructural integrity.

Methods

This prospective study included 11 patients who underwent total parathyroidectomy with heterotopic autotransplantation and cryopreservation of parathyroid tissue fragments. Part of the tissue was kept in cell culture solution at 4°C. Five time periods between 2 and 24 h were defined, and parathyroid fragments were kept in the solution for that length of time. At the end of each period, the fragments were removed from the transport solution, fixed, and prepared for ultrathin sections.

Results

Of the 11 cases studied, 10 showed ultrastructural findings consistent with cellular viability in tissue fragments that remained in the transport solution up to 12 h. Electron microscopy revealed that cell adhesion and the integrity of plasma membranes, nuclei, and mitochondria were preserved in one case for up to 24 h. Changes in mitochondrial structure represented the most constant ultrastructural damage seen in the cases studied, in addition to the presence of edema and cell vacuoles.

Conclusions

Analysis of the ultrastructure of hyperplastic parathyroid gland tissue showed that ultrastructural integrity was in most cases properly maintained in fragments stored up to 12 h in a cell culture solution at 4°C.

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Authors and Affiliations

  1. Department of Head and Neck Surgery, University of Sao Paulo Medical School, Sao Paulo, Brazil

    Carlos Eduardo Santa Ritta Barreira, Cláudio Roberto Cernea, Lenine Garcia Brandão & Fábio Luiz de Menezes Montenegro

  2. Department of Nephrology, University of Sao Paulo Medical School, Sao Paulo, Brazil

    Melani Ribeiro Custódio

  3. Department of Pathology, University of Sao Paulo Medical School, Sao Paulo, Brazil

    Elia Tamaso Espin Garcia Caldini

  4. Hospital de Base do Distrito Federal/UCCP, SMHS Área Especial QD 101, Brasília, CEP 70.330-150, Brazil

    Carlos Eduardo Santa Ritta Barreira

Authors
  1. Carlos Eduardo Santa Ritta Barreira
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  2. Cláudio Roberto Cernea
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  3. Lenine Garcia Brandão
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  4. Melani Ribeiro Custódio
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  5. Elia Tamaso Espin Garcia Caldini
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  6. Fábio Luiz de Menezes Montenegro
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Correspondence to Carlos Eduardo Santa Ritta Barreira.

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Barreira, C.E.S.R., Cernea, C.R., Brandão, L.G. et al. Effects of Time on Ultrastructural Integrity of Parathyroid Tissue Before Cryopreservation. World J Surg 35, 2440–2444 (2011). https://doi.org/10.1007/s00268-011-1261-x

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  • DOI: https://doi.org/10.1007/s00268-011-1261-x

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