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Complement and Neutrophil Function Changes After Liver Resection in Humans

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Abstract

Background

Complement activation contributes to the regulation of liver regeneration after liver resection (LR) in mice.

Methods

We hypothesized that complement activation and changes in C5a-receptors (C5aR, C5L2) on polymorphonuclear cells (PMN) and monocytes are important in clinical LR. Anaphylatoxin and C5b9 plasma levels were measured (bead-array, ELISA) (25 patients) and receptor expression was assessed after LR (19 patients) (FACS). In vitro PMN C5a-dependent chemotactic response (7 patients) as well as l-selectin shedding and Mac-1 expression (3 patients) was determined.

Results

C3a increased after LR (31.1 ± 4 before LR vs. 41.6 ± 5 ng/ml, 30 min after LR, P < 0.01), as did C5b9 (12.7 ± 1 before LR vs. 26.9 ± 3 ng/ml, 60 min after LR, P < 0.001). C4a and C5a decreased after LR, by 25% 24 h after LR and 30% 2 h after LR, respectively (P < 0.01). C5L2 expression decreased at 4 h, rising at 24 h after LR (PMN: 6.3 ± 1 before LR, 3.1 ± 1, 4 h, 8.3 ± 2, 24 h; P < 0.01). The receptor-related changes accompanied a diminished C5a-dependent chemotactic response by PMN (42.1 ± 17 before LR vs. 2.1 ± 3 4 h after LR; P < 0.01) and a reduction of activation upon C5a-R stimulation as measured by l-selectin shedding and Mac-1 expression on PMN. Changes in C5L2 expression on monocytes paralleled postoperative impairment of liver function.

Conclusions

These results indicate that complement components are released after clinical LR and subsequently PMN display altered C5a-dependent functional responses.

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Acknowledgments

The authors thank Dr. D. McClellan for her excellent editorial assistance. This article was supported in part by NIH grant DK-59422 to J. D. Lambris.

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Authors and Affiliations

  1. Department of General and Vascular Surgery, Medical School, Johann Wolfgang Goethe-University Frankfurt, Theodor-Stern-Kai 7, Frankfurt am Main, 60590, Germany

    Christoph Werner Strey, Britta Siegmund, Saskia Rosenblum, Rosa Maria Marquez-Pinilla, Elsie Oppermann & Wolf O. Bechstein

  2. Department of Traumatology, Hand and Reconstructive Surgery, University of Ulm Medical School, Ulm, Germany

    Markus Huber-Lang

  3. Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA

    John D. Lambris

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  1. Christoph Werner Strey
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  2. Britta Siegmund
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  3. Saskia Rosenblum
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  4. Rosa Maria Marquez-Pinilla
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  5. Elsie Oppermann
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Correspondence to Christoph Werner Strey.

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Strey, C.W., Siegmund, B., Rosenblum, S. et al. Complement and Neutrophil Function Changes After Liver Resection in Humans. World J Surg 33, 2635–2643 (2009). https://doi.org/10.1007/s00268-009-0209-x

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  • DOI: https://doi.org/10.1007/s00268-009-0209-x

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