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Exploring Preclinical Experiments with Different Fat Types for Autologous Fat Grafting

  • Original Articles
  • Basic Science/Experimental
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Aesthetic Plastic Surgery Aims and scope Submit manuscript

A Correction to this article was published on 04 April 2024

This article has been updated

Abstract

Background

Autologous fat transplantation has been a cornerstone of tissue regeneration for decades. However, there is no standardized selection system or criteria for fat graft selection, often relying heavily on the surgeon’s experience.

Objectives

This study aimed to investigate various types of fat derivatives, both in vitro and in vivo at the same condition.

Methods

We collected traditional fat granules of different sizes and SVF-gel, evaluating the viability of ADSCs isolated from them and their performance after grafting into mice.

Results

Large fat granules exhibited more complete adipocyte structures, and the isolated ADSCs demonstrated superior differentiation, proliferation, and secretion capacities. They also showed excellent volume retention after 12 weeks. In contrast, ADSCs isolated from SVF-gel displayed lower vitality. However, grafts from SVF-gel exhibited the highest volume maintenance rate among the four groups after 12 weeks, closely resembling normal adipose tissue and displaying significant vascularization. Compared to large fat granule and SVF-gel group, medium and small fat granule grafts exhibited lower volume retention and less angiogenesis.

Conclusions

Through preclinical studies, the flexible clinical use of different fat grafts can be tailored to their unique characteristics.

Level of Evidence I

This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

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Funding

This work was financially supported by School-level Project of Affiliated Hospital of Xuzhou Medical University(Grants 2022104922), Scientific Research Fund for Talent Introduction of Affiliated Hospital of Xuzhou Medical University (Grants 2024203005).

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Author notes

  1. Yixi Yu and Huabin Zhang have contributed equally to this work and should be considered co-first authors.

Authors and Affiliations

  1. Xuzhou Medical University, Xuzhou, Jiangsu Province, China

    Yixi Yu, Zefan Sun, Shun Wang & Xiangbin Zhao

  2. Xuzhou Central Hospital, Xuzhou, Jiangsu Province, China

    Huabin Zhang

  3. Department of Plastic and Cosmetic Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China

    Bingkun Zhao & Aijun Zhang

Authors
  1. Yixi Yu
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  2. Huabin Zhang
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  4. Shun Wang
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  5. Xiangbin Zhao
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  6. Bingkun Zhao
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  7. Aijun Zhang
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Corresponding authors

Correspondence to Bingkun Zhao or Aijun Zhang.

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The authors declare no conflicts of interest to disclose.

Ethical Approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. The adipose tissues were obtained from 12 young women (aged 20–35 years, weighing 50–70 kg) after lower abdominal liposuction at the Plastic and Cosmetic Center of the Affiliated Hospital of Xuzhou Medical University, and the surgical operations were all performed by the same surgeon. Local skin infections, infectious diseases, hypertension, diabetes, and other diseases were excluded, and the purpose of the experiment was informed before the operation, informed consent was signed, and the operation was approved by the hospital ethics committee. All applicable institutional and/or national guidelines for the care and use of animals were followed. The experimental animals were 30 SPF-grade BALB/c nude mice provided by the Animal Experiment Center of Xuzhou Medical University, License No.: SCXK (Su) 2018-0008, 4–6 weeks of age, weighing about 20 g. The experimental operations were reviewed and approved by the University Ethics Committee.

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Aijun Zhang and Bingkun Zhao are co-corresponding authors of this article.

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Yu, Y., Zhang, H., Sun, Z. et al. Exploring Preclinical Experiments with Different Fat Types for Autologous Fat Grafting. Aesth Plast Surg (2024). https://doi.org/10.1007/s00266-024-03905-6

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  • DOI: https://doi.org/10.1007/s00266-024-03905-6

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