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Liquid Phase Concentrated Growth Factor Improves Autologous Fat Graft Survival In Vivo in Nude Mice

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  • Basic Science/Experimental
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Abstract

Objective

The present study aimed to explore the efficacy and safety profile of liquid phase concentrated growth factor (LPCGF) in promoting autologous fat graft survival.

Methods

LPCGF/PRP was mixed with human fat tissues at different proportions and transplanted into nude mice. Three months after transplantation, the implanted fat tissues were retrieved for analysis. H&E staining was used to quantify the neovascularization. Immunohistochemical staining was applied to quantify the CD34-positive stem cells and the fluorescence intensity of VEGF and TGF-β.

Results

Addition of LPCGF to autologous fat reduced the fat absorption by 5–15%, especially at the early stage, and no complications were observed. In addition, the effect was improved with increased CGF. Liquid phase concentrated growth factor improves autologous fat graft survival, and the most suitable ratio of LPCGF/fat is 1:8.

Conclusion

LPCGF is rich in VEGF, TGF-β and CD34-positive stem cells, which can improve the fat transplantation effect, but the specific influence of a single component requires future evaluation.

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Abbreviations

LPCGF:

Liquid phase concentrated growth factor

PRP:

Platelet-rich plasma

HSCs:

Hematopoietic stem cells

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Acknowledgements

We would like to acknowledge the reviewers for their helpful comments on this paper.

Funding

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Authors and Affiliations

  1. Shanghai Ninth People’s Hospital, Department of Oral and Craniomaxillofacial Surgery, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, 639 Meiji Road, Huangpu District, Shanghai, 200011, China

    Tianjia Zhang, Jiewen Dai, Yuting Xu, Liya Yu & Xudong Wang

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  1. Tianjia Zhang
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  2. Jiewen Dai
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  3. Yuting Xu
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  4. Liya Yu
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  5. Xudong Wang
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Contributions

XW finished study design, TZ, YX finished experimental studies, JD, LY finished data analysis, TZ, JD finished manuscript editing. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Xudong Wang.

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Conflict of interest

The authors declare that they have no conflict of interest.

Human and Animal Rights

This study was approved and supervised by the animal ethics committee of National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology. The treatment of animals in all experiments conforms to the ethical standards of experimental animals.

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Tianjia Zhang and Jiewen Dai are co-first authors.

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Zhang, T., Dai, J., Xu, Y. et al. Liquid Phase Concentrated Growth Factor Improves Autologous Fat Graft Survival In Vivo in Nude Mice. Aesth Plast Surg 45, 2417–2422 (2021). https://doi.org/10.1007/s00266-021-02336-x

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  • DOI: https://doi.org/10.1007/s00266-021-02336-x

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