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Dual Network Hydrogels Based on PRP and SA Promote the Retention Rate and Vascularization of Transplanted Fat

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  • Fat Injection
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Abstract

Background

Autologous adipose tissue often experiences ischemia and hypoxia after transplantation, leading to low retention rates and unstable operative impacts due to necrotic absorption. Platelet-rich plasma (PRP) can enhance fat regeneration and increase the fat retention rate after transplantation. However, the quick release of growth factors (GFs) in PRP decreases therapeutic efficiency. This study aimed to achieve a slow release of PRP to promote fat retention.

Methods

We prepared a dual-network hydrogel (DN gel) based on FDA-approved PRP and sodium alginate (SA) through a simple “one-step” activation process. In vivo study, adipose tissue with saline (control group), SA gel (SA gel group), PRP gel (PRP gel group), and DN gel (DN gel group) was injected subcutaneously into the dorsum of nude mice. At 4 and 12 weeks after injection, tissues were assessed for volume and weight. Hematoxylin and eosin staining (HE) and immunofluorescence staining were performed for histological assessment.

Results

DN gel exhibits long-lasting growth factor effects, surpassing conventional clinical PRP gel regarding vascularization potential. In fat transplantation experiments, DN gel demonstrated improved vascularization of transplanted fat and increased retention rates, showing promise for clinical applications.

Conclusions

DN gel-assisted lipofilling can significantly improve the retention rate and quality of transplanted fat. DN gel-assisted lipofilling, which is considered convenient, is a promising technique to improve neovascularization and fat survival.

No Level Assigned

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Acknowledgement

Thanks for all the contributors.

Funding

This research was supported by the Military Logistics Scientific Research Program of China (BKJ18J003) and the General Project of Natural Science Basic Research Program of Zhejiang Province (82272296).

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, China

    Huichen Li, Han Peng, Baoyan Liang, Zhaoxiang Zhang & Chenggang Yi

  2. The College of Life Science, Northwest University, Xi’an, 710069, China

    Huichen Li, Han Peng & Jiezhang Tang

  3. School of Basic Medicine, Fourth Military Medical University, Xi’an, 710032, China

    Huichen Li, Yun Zhu, Yi Cheng & Lihua Yang

  4. Department of Plastic and Burn Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, 710038, China

    Jiezhang Tang

  5. Department of Dermatology, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061, China

    Mengmeng Hou

  6. Department of Plastic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China

    Chenggang Yi

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  1. Huichen Li
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  2. Han Peng
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Correspondence to Chenggang Yi.

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Conflict of interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Ethical Approval

The work described has not been submitted elsewhere for publication. All authors have seen and approved the final version of the manuscript being submitted.

Human and Animal Rights

All animal experiments were approved by the Ethics Committee of the Fourth Military Medical University (No. IACUC-20211250). Human tissue collection involved in this research were approved by the Ethics Committee of the Fourth Military Medical University (No. KY20213150-1).

Informed Consent

Informed consent was obtained from all tissue donors.

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Li, H., Peng, H., Tang, J. et al. Dual Network Hydrogels Based on PRP and SA Promote the Retention Rate and Vascularization of Transplanted Fat. Aesth Plast Surg 48, 501–509 (2024). https://doi.org/10.1007/s00266-023-03783-4

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  • DOI: https://doi.org/10.1007/s00266-023-03783-4

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