After surgical treatment of high-grade soft tissue sarcomas, local recurrences, metastases and survival remain a great concern. Further knowledge on factors with a possible impact on these endpoints, specifically resection margins, is relevant for decision-making regarding the aggressiveness of local treatment. The aim of this study is to investigate the impact of prognostic factors on local recurrence and overall survival for patients with high-grade soft tissue sarcomas of the extremities.
In a retrospective cohort study of 127 patients (mean age 48 years, range five to 91; median follow-up 71 months) the prognostic effect of margin status and other clinicopathologic characteristics on local recurrence and overall survival were analysed by employing a multivariate Cox regression.
Five-year cumulative incidence of local recurrence and distant metastases was 26 % and 40 %, respectively. The estimated five-year overall survival was 59 %. Tumour size proved a consistent adverse prognostic factor for local recurrence (hazard ratio (HR) 3.9), distant metastasis (HR 4.9) and overall survival (HR 2.4). The significant association of resection margins with local recurrence (HR 10.2) was confirmed. Margins were however not significantly associated with the occurrence of distant metastasis or overall survival. The occurrence of local recurrence had a significant impact on overall survival (HR 2.0).
The results of this study confirm the critical role of tumour size on survival and margins on local recurrence, and stress the need for further investigation concerning the association between margins, local recurrence and survival.
Soft tissue sarcomas (STS) are a rare (incidence 1:30.000) heterogeneous group of tumours accounting for approximately 1 % of all adult cancers . Despite continuous improvements in imaging, surgical techniques and adjuvant treatment, local recurrences, distant metastasis and death remain of great concern especially in high-grade tumours. Five-year cumulative incidence rates range from 12 to 28 % for local recurrence and 21–40 % for distant metastasis [2–6]. Defining a consistent set of prognostic variables for these oncologic end-points has proven challenging due to the heterogeneity and low incidence of high-grade tumours (≈38 % of all STS) . Some factors have consistently proven to be predictive of adverse events, such as histotype, depth, histological grade, and size [2–11]. The role of resection margins has also been extensively studied in highly heterogeneous sarcoma populations. There is general agreement that positive margins are associated with a higher risk of local recurrence [4–6, 8–11]. The association between resection margins and distant metastasis or overall survival however remains unclear [3, 4, 6, 8–11]. The majority of studies addressing this issue have included all histologic grades and sarcoma types leading to general outcomes, which are intricate to use in a clinical setting. Questioning the effect of margins and local recurrences on survival remains relevant, as it influences the extent of local and adjuvant treatment. This study focuses only on STS of high grade to improve understanding of this subgroup of unfavourable STS that is more homogeneous in the context of survival outcomes.
A competing risks model was used to estimate the cumulative incidence of local recurrence and distant metastasis. When the situation is such that the occurrence of an event (e.g., death) prevents the event of interest (e.g., local recurrence) from happening, a competing risks model must be applied . If this analysis is not used, the cumulative incidence rate (the probability of failure from a specific cause) is generally overestimated.
The objective of this study was to evaluate the effect of margins, adjusted for other known risk factors, on local recurrence, distant metastases, and overall survival.
Patients and methods
A retrospective analysis of patients surgically treated between 2000 and 2007 at our two tertiary referral centres for primary, high-grade, STS of the extremities (ESTS) was performed. From the pathology databases 161 patients with one of four most common high-grade ESTS diagnoses—high-grade myxofibrosarcoma, synovial sarcoma, malignant peripheral nerve sheath tumour, or angiosarcoma—were identified. Exclusion criteria were metastatic disease at time of diagnosis, presentation with recurrent disease, no operative treatment with curative intent at our institutions, and an unknown margin status. All patients had pre-treatment staging with a lung CT-scan to rule out the presence of metastasis. Thirty-four patients were excluded from analysis (presentation with metastases (11), recurrent disease (16), no operative treatment (7)) resulting in a cohort of 127 patients (mean age at time of diagnosis: 48 years (range five to 91), including nine children; male/female ratio 1:0.87).
Medical records including operative and pathologic reports were reviewed and the following information was recorded: age, gender, presentation status (no treatment/biopsy only vs. prior (Whoops) excision), tumour size (small (<50 mm) vs. intermediate (50–100 mm) vs. large (≥100 mm)), depth (superficial vs. deep to investing fascia), location (upper vs. lower extremity), involvement of adjacent structures, surgical margin, adjuvant therapy (radiotherapy vs. no radiotherapy; chemotherapy vs. no chemotherapy), and follow-up data. Table 1 summarizes patient, tumour and treatment characteristics.
Independent experienced musculoskeletal pathologists (PH, JB, AF) defined the closest surgical margin after inking and sectioning of the specimen. The closest identified resection margin was categorized as follows: intralesional for a positive margin with tumour cells visible at the inked margin (12 %), marginal for a margin of 2 mm or less (58 %), or wide for a negative margin of more than 2 mm (30 %) . The decision concerning the use of adjuvant treatment was not uniform during the study period due to progressing insights in the lapse of time, although nearly all patients (80 %) received radiotherapy. The most common regimen was 50Gy on the target region and a boost of 10Gy on the tumour region. Adjuvant therapy was omitted in 24 (19 %) patients for various reasons (amputation, patient preference (seven patients), superficial tumours (seven tumours) or wide resection margins (12 patients)). No other adjuvant therapies, besides chemotherapy or radiotherapy, were administered.
Local recurrence was defined as the first clinical, radiological or pathological manifestation of tumour of the same histologic type within or contiguous to the previously treated tumour bed, one or more months after primary treatment. Distant recurrence was defined by clinical or radiological evident systemic spread of tumour outside the primary tumour bed, including nodal metastasis. Dates of death were extracted from the medical records, which were coupled to the general registry of the hospital.
The outcomes of interest were five year cumulative incidence of local recurrence and distant metastasis, and five year overall survival rate. Synchronous local and distant recurrences were considered as separate events in the analysis of local recurrence and distant metastasis. Time to recurrence and time to death were estimated from date of first surgery (excluding previous Whoops excisions). Survival end-point was date of last follow-up or death. The median follow-up, as assessed by employing the reverse Kaplan-Meier method , was 71 months (95 % CI 62–80).
The following variables were tested for univariate association with local-recurrence, distant-recurrence and survival using the log rank test statistic: age, gender, anatomic site, tumour growth, depth, previous Whoops excision, planned primary amputation, margin, histological subtype, and adjuvant radio- or chemotherapy. Factors identified as significant were included in the multivariate analysis. For local recurrence and distant metastasis a cause-specific multivariate hazard Cox regression model was applied. The cause-specific hazard function is the fundamental concept in the competing risks model; it provides the hazard of failing from a specific cause in the presence of the competing events . For overall survival a Cox model with local and distant recurrences as time-dependent covariates was estimated. P values at or below 0.05 were considered significant. To check the proportional hazards assumption we looked at the Shoenfeld residuals. Statistical analyses were performed in SPSS (version 20; IBM Corp, Armonk, NY) and R 2–18 . All analyses concerning competing risks were performed with the MState library .
Disease outcome and patient survival
Five year cumulative incidence of local recurrence and distant metastases was 26 % and 40 %, respectively. The estimated five-year overall survival was 59 % with a median survival time of 79 months (95 % CI, 65–93 months) (Fig. 1).
Among patients with intralesional margins, six (50 %) and seven (58 %) patients developed a local recurrence and/or distant metastasis, respectively. This was the case for 22 (30 %) and 32 (43 %) patients with marginal margins, and three (8 %) and 11 (29 %) patients with wide margins. Eventually, seven (47 %), 37 (50 %), and 14 (47 %) patients with intralesional, marginal, or wide margins, respectively, died.
Table 2 shows the results from the univariate and multivariate analyses for all endpoints.
Univariate prognostic factors for local recurrence were margin status, tumour size, and age. For distant metastases, tumour size and a presentation after ‘Whoops’ surgery were significant factors. Tumour size, age and a primary limb sparing operation (vs. amputation) were significant factors for overall survival. Radiotherapy did not emerge as a significant univariate prognostic factor for either endpoint; merely a slight trend towards better survival was observed.
In the multivariate analysis, prognostic factors for local recurrence were margins and tumour size. Patients with intralesional and marginal resection margins had 10.2 times (95 % CI, 2.5–42.4) and 4.8 times (95 % CI, 1.4–16.2) higher risk (95 % CI, 1.4–16.2) of experiencing local recurrence, respectively. Patients presenting with large tumours (≥100 mm) had 3.9 times higher risk (95 % CI, 1.5–10.4) of developing a local recurrence compared to patients with small (<50 mm) tumours. In Figs. 2 and 3 the cumulative incidence curves of local recurrence are represented for resection margin and tumour size respectively. For distant metastases, tumour size remained the only prognostic factor associated with the occurrence of distant metastases (hazard ratio 4.9 (95 % CI, 1.9–12.4) and 2.4 (95 % CI, 0.9–6.2)) for large and intermediate tumours, respectively). No correlation between tumour size and surgical margins could be observed (Table 3). To assess the impact of risk factors on overall survival a Cox model with local recurrence as time-dependent variable has been estimated. Independent significant factors were size and presence of local recurrence. The hazard ratio for tumour size was equal to 2.4 (95 % CI, 1.1–2.9) and 1.1 (95 % CI, 0.5–2.5) for patients with large or intermediate tumours, respectively, compared to patients with small tumours. Development of a local recurrence was associated with a 2.0-fold (95 % CI, 1.1–3.7) increased risk of death. A Cox model with distant metastasis as time-dependent covariate was also employed. As expected the hazard ratio associated with distant metastasis was extremely high, outweighing all other prognostic variables. As a result none showed a significant statistical effect. The risk of death increased 17-fold with a distant metastasis (95 % CI, 8.6–33.3).
Surgery remains the cornerstone of treatment for patients with ESTS. Although local control is one of the primary objectives of treatment, the question is whether this has to be sought for at every extent, leading to possibly unacceptable functional outcomes. The lack of unambiguous evidence that margins and local control influence survival further complicates decision-making. Moreover, results from many studies are confounded by the amount of histological subtypes included. Therefore this study aimed to define the influence of the extent of local treatment (i.e., margins) in the light of other prognostic factors on local recurrence and survival in a specific group of high-grade ESTS.
The cumulative incidences of local recurrence and distant metastases at five years were 26 % and 40 %, respectively. Five-year overall survival was 59 %. These results are somewhat less optimistic than those previously published, although a comparison is difficult due to differences in the patient population (high grade vs. all STS subtypes) and statistical methods [2, 17, 18]. As expected, tumour size was a consistent adverse prognostic risk factor for the occurrence of local recurrence, distant metastasis, and overall survival, in agreement with published results [4, 6, 11, 19].
In this series, intralesional and marginal margins had a significant effect on the development of local recurrences, while the development of distant metastases and overall survival were not significantly affected by the surgical margin. This apparent controversial manifestation has been reported previously, although a clear explanation is still lacking. Generally it is hypothesized that these results can be explained on the basis of tumour-specific or biologic factors, with highly aggressive tumours leading to greater risk of distant spread and subsequent death irrespective of local surgery [4, 8, 9, 17, 20]. Following from this, the question of the prognostic role of local recurrence arises. In this study, the occurrence of local recurrence was associated with an increased risk of death, as shown in several studies [2, 13, 10, 19], while others show no association between local recurrence and overall survival [9, 21, 22]. As to the cause of the increased risk we can merely hypothesize between a direct effect of local recurrence on survival and an indirect effect with local recurrence as a marker of high biological tumour aggressiveness. The latter would implicate that the described relationship between local recurrences and overall survival is a result of selection bias. The outcomes of this study reflect the unresolved discussion, with local recurrence as an adverse prognostic factor for survival on one hand, while the lack of a relationship between surgical margins and overall survival argues for local recurrence as a marker of tumour aggressiveness.
When evaluating the effect of margin status, the inconsistencies in definitions of positive margins are an obvious problem. Most investigations classify margin as positive (R1) or negative (R0) margins, because application of Enneking criteria is hampered in retrospective studies. A solely positive or negative status of a margin however, gives no insight into the difference between clear but close margins and wider margins. Exactly this distinction is important in the light of sparing as much tissue as possible for preferable functional outcomes . With improvements in preoperative planning using MRI and the use of (neo) adjuvant therapy, sufficient margins have gotten evermore narrow and the classification of a wide margin as >2 mm is more in line with the reality of current deep, high-grade STS resections .
Due to the presence of a competing risk (death) precluding an event of interest (local recurrence, metastasis), this study applies the competing risk model. In the presence of competing risks, the Kaplan–Meier method generally overestimates the risk of the event at interest, because it assumes that patients who experienced the competing event are still at risk for the event of interest and thus cumulative probabilities are overestimated . We feel it needs to be noted that many studies, upon which clinical decisions are made, simply overlook the competing risk situation.
Some limitations are present in our study. First of all, due to the low incidence of these sarcomas our sample size is small. This might account for the fact that some expected prognostic factors, such as age or limb sparing procedure, were not significant in our analysis. The second aspect concerns the retrospective nature of the study where selection bias and confounding cannot be excluded. Due to the multicentre aspect of the study the pathologic specimens were neither evaluated by the same pathologist nor were they reviewed to ensure consistency in reporting. Finally, the lack of standardization of adjuvant therapy is a limitation. It has been well established that adjuvant radiotherapy improves local control of STS. The impact on overall survival has however not been established . An attempt to further analyse this aspect was not made in this study. However, there were no significant differences in outcome by any of the adjuvant treatment parameters, except a slight trend of better survival for those receiving adjuvant radiotherapy. In this study 80 % of the patients received adjuvant treatment, thus this minor trend may likely be based on selection bias.
This study shows that tumour size is a consistent adverse prognostic factor for local recurrence, distant metastasis, and overall survival. In addition, our data show a correlation between resection margins and the occurrence of local recurrence. This correlation was not observed for the occurrence of distant metastases or overall survival, while simultaneously a trend of an increased risk of death after local recurrence was noted. The results of this study emphasize once more the need for further convincing evidence of the influence of margins and local recurrence on survival in high-grade ESTS, while stressing the importance of early diagnosis and treatment, with tumour size as the most important prognostic factor.
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The authors gratefully acknowledge Prof. Pancras Hoogendoorn (PH) and Prof. Judith Bovee (JB) of the Leiden University Medical Center, and Prof. Adrienne Flanagan (AF) of the Royal National Orthopedic Hospital, for their (histo) pathological expertise.
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Willeumier, J., Fiocco, M., Nout, R. et al. High-grade soft tissue sarcomas of the extremities: surgical margins influence only local recurrence not overall survival. International Orthopaedics (SICOT) 39, 935–941 (2015). https://doi.org/10.1007/s00264-015-2694-x