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On-line enzyme activity determination using the stopped-flow technique: application to laccase activity in pulp mill waste-water treatment

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Abstract

An automated system for on-line measurement of enzyme activity is proposed. The system uses a flow injection manifold in the stopped-flow mode to measure initial reaction rates. The time during which the flow is halted is selected in such a way as to optimise the enzyme/substrate ratio for the correct determination of activity values. The proposed system was used to determine the activity of laccase produced by the fungus Trametes versicolor immobilised on nylon in a fixed-bed reactor used for treating pulp mill waste water.

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Authors and Affiliations

  1. Departament d'Enginyeria Química, Facultat de Ciències, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain Tel.: (3) 581 18 79 Fax: (3) 581 20 13 e-mail: Font@uab-eq.uab.es, , , , , , ES

    X. Font, X. Gabarrell, J. Lafuente & M. T. Vicent

  2. Laboratorio Asociado al Centro Nacional de Biotechnologia (UAB-CSIC), Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain, , , , , , ES

    G. Caminal

Authors
  1. X. Font
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  2. G. Caminal
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  3. X. Gabarrell
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  4. J. Lafuente
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  5. M. T. Vicent
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Additional information

Received: 17 February 1997 / Received revision: 23 April 1997 / Accepted: 27 April 1997

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Font, X., Caminal, G., Gabarrell, X. et al. On-line enzyme activity determination using the stopped-flow technique: application to laccase activity in pulp mill waste-water treatment. Appl Microbiol Biotechnol 48, 168–173 (1997). https://doi.org/10.1007/s002530051033

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  • DOI: https://doi.org/10.1007/s002530051033

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