Abstract
Large-scale exploitation of the potential benefits of ectomycorrhizal fungi in improving plantation yields means that fermentation techniques for these fungi will be required. Starting with a base performance on a rich, complex medium, the effect of variations in some physicochemical culture parameters on biomass yield was studied. It was possible to reduce the amount of phosphate salts (to 1/9th) and other ingredients (to 1/3rd) in the medium. A shaking speed of either 100 rpm or 200 rpm in an orbital incubator was satisfactory and biomass yield responded to an increase in carbon substrate (glucose, from 10 g l-1 and 20 g l-1) though Y x/s declined. An increase in inoculum size shortened culture time but decreased biomass yield. The upper limit of the incubation temperature was between 25°C and 30°C. Biomass yields were about 12 g l-1 dry weight (Y x/s=0.63) when 20 g l-1 glucose was supplied, and about 7 g l-1 (Y x/s=0.74) when 10 g l-1 glucose was supplied.
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Received: 9 October 1995/Accepted: 4 December 1995
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Kuek, C. Shake-flask culture of Laccaria laccata, an ectomycorrhizal basidiomycete. Appl Microbiol Biotechnol 45, 319–326 (1996). https://doi.org/10.1007/s002530050690
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DOI: https://doi.org/10.1007/s002530050690