Inorganic polyphosphate in methylotrophic yeasts

Andreeva, Nadeshda; Ryazanova, Lubov; Zvonarev, Anton; Trilisenko, Ludmila; Kulakovskaya, Tatiana; Eldarov, Mikhail

doi:10.1007/s00253-018-9008-3

Applied microbial and cell physiology
Published: 21 April 2018

Inorganic polyphosphate in methylotrophic yeasts

Nadeshda Andreeva¹,
Lubov Ryazanova¹,
Anton Zvonarev¹,
Ludmila Trilisenko¹,
Tatiana Kulakovskaya¹ &
Mikhail Eldarov²

Applied Microbiology and Biotechnology volume 102, pages 5235–5244 (2018)Cite this article

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Abstract

Inorganic polyphosphate (polyP) is a significant regulatory and metabolic compound in yeast cells. We compared polyP content and localization, polyphosphatase activities, and transcriptional profile of polyP-related genes in industrially important methylotrophic yeasts, Hansenula polymorpha and Pichia pastoris. The increased need for phosphate, the decrease of long-chain polyP level, the accumulation of short-chain polyP, and enhanced endopolyphosphatase activity in the crude membrane fraction were observed in methanol-grown cells compared with glucose-grown cells of both species. Transcriptome analysis revealed notable differences in the expression patterns of key genes encoding proteins related to polyP metabolism. In methanol-grown cells, the genes encoding endopolyphosphatases and phosphate transporters were upregulated. The changes in polyP metabolism are probably related to the peculiarities of bioenergetics of methanol-grown cells.

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Acknowledgements

The authors thank Elena Makeeva for her help with preparing the manuscript.

Funding

This study was funded by the Russian Foundation for Basic Research (Grant No. 17-04-00822).

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Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, 142290, Russia
Nadeshda Andreeva, Lubov Ryazanova, Anton Zvonarev, Ludmila Trilisenko & Tatiana Kulakovskaya
FRC Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, pr. Shestidesyatiletiya Oktyabrya 7-1, Moscow, 117312, Russia
Mikhail Eldarov

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Nadeshda Andreeva
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Lubov Ryazanova
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Anton Zvonarev
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Ludmila Trilisenko
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Tatiana Kulakovskaya
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Mikhail Eldarov
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Correspondence to Tatiana Kulakovskaya.

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Andreeva, N., Ryazanova, L., Zvonarev, A. et al. Inorganic polyphosphate in methylotrophic yeasts. Appl Microbiol Biotechnol 102, 5235–5244 (2018). https://doi.org/10.1007/s00253-018-9008-3

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Received: 07 November 2017
Revised: 05 April 2018
Accepted: 07 April 2018
Published: 21 April 2018
Issue Date: June 2018
DOI: https://doi.org/10.1007/s00253-018-9008-3

Keywords

Polyphosphate
Polyphosphatase
Methylotrophic yeast
Hansenula polymorpha
Pichia pastoris
Transcriptome