A novel cell autolysis system for cost-competitive downstream processing
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The industrial production of low value-added biological products poses significant challenges due to cost pressures. In recent years, it has been argued that synthetic biology approaches will lead to breakthroughs that eliminate price bottlenecks for the production of a wide range of biological products including bioplastics and biofuels. One significant bottleneck lies in the necessity to break the tough cell walls of microbes in order to release intracellular products. We here report the implementation of the first synthetic biology standard part based on the lambda phage SRRz genes and a synthetic ribosome binding site (RBS) that works in Escherichia coli and Halomonas campaniensis, which enables the producer strains to induce lysis after the addition of small amounts (1–5 %) of solvents or to spontaneously lyse during the stresses of downstream processing, and thus has the potential to eliminate the mechanical cell disruption step as both an efficiency bottleneck and a significant capex barrier when implementing downstream bioprocesses.
KeywordsAutolysis PHB PHA Bioplastics Halomonas Synthetic biology
Plasmid pSEVA311 was kindly donated by Prof. Victor de Lorenzo of Spanish National Centre of Biotechnology (CSIC, CNB, Syst Biol Program). This research was financially supported by 973 Basic Research Fund (Grant No. 2012CB725201) and a grant from National Natural Science Foundation of China (Grant No. 31430003).
Ivan Hajnal devised the experiments. Ivan Hajnal and Xiangbin Chen conducted the experiments. Guo-Qiang Chen supervised the studies and revised the manuscript.
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Li R, Zhang HX, Qi QS (2007) The production of polyhydroxyalkanoates in recombinant Escherichia coli. Appl Microbiol Biotechnol 98:2313–2320Google Scholar
- Silva-Rocha R, Martínez-García E, Calles B, Chavarría M, Arce-Rodríguez A, de las Heras A, Platero R, de Lorenzo V (2013) The standard European vector architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Res 41:666–675CrossRefGoogle Scholar
- Young RY (2002) Bacteriophage holins: deadly diversity. J Mol Microbiol 4:21–36Google Scholar