Abstract
In order to produce rubusoside, enzymes with preferential specificity for the saccharide sophorose were tested for ability to produce rubusoside from stevioside. We identified BGL1, a β-glucosidase from Streptomyces sp. GXT6, as an enzyme for rubusoside production. Out of several saccharide substrates, BGL1 showed the most affinity to sophorose. This enzyme only hydrolyzes the glucose moiety of the sophoroside at C-13 in stevioside. Production of rubusoside was determined by 1H and 13C nuclear magnetic resonance (NMR). Thus, rubusoside was produced from stevioside and the stevioside conversion rate was 98.2 %. The production yield of rubusoside was 78.8 % in 6 h.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 31360369, 21366007), the Innovation Project of Guangxi Graduate Education (No. YCBZ2015005) and the Natural Science Foundation of Guangxi (No. 2014GXNSFAA118097).
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Wang, Z., Wang, J., Jiang, M. et al. Selective production of rubusoside from stevioside by using the sophorose activity of β-glucosidase from Streptomyces sp. GXT6. Appl Microbiol Biotechnol 99, 9663–9674 (2015). https://doi.org/10.1007/s00253-015-6802-z
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DOI: https://doi.org/10.1007/s00253-015-6802-z