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Effects of antimicrobial peptide L-K6, a temporin-1CEb analog on oral pathogen growth, Streptococcus mutans biofilm formation, and anti-inflammatory activity

  • Applied microbial and cell physiology
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Abstract

Dental caries and periodontitis are common bacterial mouth infections. As a potentially attractive substitute for conventional antibiotics, antimicrobial peptides have been widely tested and used for controlling bacterial infections. In this study, we tested the efficacy of the peptides from the skin secretions of Rana chensinensis for killing several major cariogenic and periodontic pathogens as well as Candida albicans. L-K6, a temporin-1CEb analog, exhibited high antimicrobial activity against the tested oral pathogens and was able to inhibit Streptococcus mutans biofilm formation and reduce 1-day-old S. mutans biofilms with a minimum biofilm inhibitory concentration and reducing concentration of 3.13 and 6.25 μM, respectively. The results of confocal laser scanning microscopy demonstrated that the peptide significantly reduced cell viability within oral biofilms. Furthermore, as little as 5 μM L-K6 significantly inhibited lipopolysaccharide (LPS)- and interleukin-1β-induced productions of interleukin-8 and tumor necrosis factor-α from THP-1 monocytic cells. This anti-inflammatory activity is associated with the binding of L-K6 to LPS and neutralizing LPS-induced proinflammatory responses in THP-1 cells, as well as dissociating LPS aggregates. Our results suggest that L-K6 may have potential clinical applications in treating dental caries by killing S. mutans within dental plaque and acting as anti-inflammatory agents in infected tissues.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 31272314 and No. 81202448), the Natural Science Foundation of Liaoning (Grant No. 201202121), and the Program for Liaoning Innovative Research Team in University (LT2012019).

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Authors and Affiliations

  1. Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Liaoning Normal University, Dalian, 116081, People’s Republic of China

    Dejing Shang, Hao Liang, Shi Wei, Xin Yan, Qingzu Yang & Yue Sun

  2. Faculty of Life Science, Liaoning Normal University, Dalian, 116081, People’s Republic of China

    Dejing Shang

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  1. Dejing Shang
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  2. Hao Liang
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  3. Shi Wei
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  4. Xin Yan
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  5. Qingzu Yang
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  6. Yue Sun
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Correspondence to Dejing Shang.

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Shang, D., Liang, H., Wei, S. et al. Effects of antimicrobial peptide L-K6, a temporin-1CEb analog on oral pathogen growth, Streptococcus mutans biofilm formation, and anti-inflammatory activity. Appl Microbiol Biotechnol 98, 8685–8695 (2014). https://doi.org/10.1007/s00253-014-5927-9

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  • DOI: https://doi.org/10.1007/s00253-014-5927-9

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