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Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 improves acute liver injury induced by d-galactosamine in rats

  • Applied microbial and cell physiology
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Abstract

This work investigated the effect of the intragastric administration of five lactic acid bacteria from healthy people on acute liver failure in rats. Sprague–Dawley rats were given intragastric supplements of Lactobacillus salivarius LI01, Lactobacillus salivarius LI02, Lactobacillus paracasei LI03, Lactobacillus plantarum LI04, or Pediococcus pentosaceus LI05 for 8 days. Acute liver injury was induced on the eighth day by intraperitoneal injection of 1.1 g/kg body weight d-galactosamine (d-GalN). After 24 h, samples were collected to determine the level of liver enzymes, liver function, histology of the terminal ileum and liver, serum levels of inflammatory cytokines, bacterial translocation, and composition of the gut microbiome. The results indicated that pretreatment with L. salivarius LI01 or P. pentosaceus LI05 significantly reduced elevated alanine aminotransferase and aspartate aminotransferase levels, prevented the increase in total bilirubin, reduced the histological abnormalities of both the liver and the terminal ileum, decreased bacterial translocation, increased the serum level of interleukin 10 and/or interferon-γ, and resulted in a cecal microbiome that differed from that of the liver injury control. Pretreatment with L. plantarum LI04 or L. salivarius LI02 demonstrated no significant effects during this process, and pretreatment with L. paracasei LI03 aggravated liver injury. To the best of our knowledge, the effects of the three species—L. paracasei, L. salivarius, and P. pentosaceus—on d-GalN-induced liver injury have not been previously studied. The excellent characteristics of L. salivarius LI01 and P. pentosaceus LI05 enable them to serve as potential probiotics in the prevention or treatment of acute liver failure.

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Acknowledgments

This study was supported by the National Basic Research Program of China (973 program) (No. 2013CB531401), the Key Program of the National Natural Science Foundation of China (No. 81330011), and the Scientific Research Fund of the Department of Education of Zhejiang Province (Y200805384).

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Authors and Affiliations

  1. State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, People’s Republic of China

    Long-Xian Lv, Xin-Jun Hu, Gui-Rong Qian, Hua Zhang, Hai-Feng Lu, Bei-Wen Zheng, Li Jiang & Lan-Juan Li

  2. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China

    Long-Xian Lv, Xin-Jun Hu, Gui-Rong Qian, Hua Zhang, Hai-Feng Lu, Bei-Wen Zheng & Lan-Juan Li

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  1. Long-Xian Lv
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  2. Xin-Jun Hu
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  3. Gui-Rong Qian
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  4. Hua Zhang
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  5. Hai-Feng Lu
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  6. Bei-Wen Zheng
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  7. Li Jiang
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  8. Lan-Juan Li
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Correspondence to Lan-Juan Li.

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Long-Xian Lv, Xin-Jun Hu, and Gui-Rong Qian contributed equally to this work.

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Lv, LX., Hu, XJ., Qian, GR. et al. Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 improves acute liver injury induced by d-galactosamine in rats. Appl Microbiol Biotechnol 98, 5619–5632 (2014). https://doi.org/10.1007/s00253-014-5638-2

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  • DOI: https://doi.org/10.1007/s00253-014-5638-2

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