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Zymogram profiling of superoxide dismutase and catalase activities allows Saccharomyces and non-Saccharomyces species differentiation and correlates to their fermentation performance

  • Applied microbial and cell physiology
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Abstract

Aerobic organisms have devised several enzymatic and non-enzymatic antioxidant defenses to deal with reactive oxygen species (ROS) produced by cellular metabolism. To combat such stress, cells induce ROS scavenging enzymes such as catalase, peroxidase, superoxide dismutase (SOD) and glutathione reductase. In the present research, we have used a double staining technique of SOD and catalase enzymes in the same polyacrylamide gel to analyze the different antioxidant enzymatic activities and protein isoforms present in Saccharomyces and non-Saccharomyces yeast species. Moreover, we used a technique to differentially detect Sod1p and Sod2p on gel by immersion in NaCN, which specifically inhibits the Sod1p isoform. We observed unique SOD and catalase zymogram profiles for all the analyzed yeasts and we propose this technique as a new approach for Saccharomyces and non-Saccharomyces yeast strains differentiation. In addition, we observed functional correlations between SOD and catalase enzyme activities, accumulation of essential metabolites, such as glutathione and trehalose, and the fermentative performance of different yeasts strains with industrial relevance.

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Acknowledgments

We are grateful to Lallemand SL for transferring of non-commercial strains from their collection, and to Dr. A. Querol for the gift of the S. kudriavzevii, the S. paradoxus and the S. c × S. k hybrid strains. This work has been supported by grants AGL2008-00060 and AGL2011-24353 from the Spanish Ministry of Education and Science (MEC) to E.M, and it has been performed within the Programme VLC/Campus, Microcluster IViSoCa (Innovation for a Sustainable Viticulture and Quality), and Microcluster BBLM (Model Yeasts in Biomedicine & Biotechnology). E.G.-S. is a predoctoral fellow of the JAE program from the CSIC (Spanish National Research Council). R.G.-P. was a predoctoral fellow of the I3P program from the CSIC.

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Authors and Affiliations

  1. Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Valencia, Spain

    Esther Gamero-Sandemetrio & Emilia Matallana

  2. Departament de Bioquímica i Biología Molecular, Universitat de València, València, Spain

    Rocío Gómez-Pastor & Emilia Matallana

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  1. Esther Gamero-Sandemetrio
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  2. Rocío Gómez-Pastor
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  3. Emilia Matallana
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Correspondence to Emilia Matallana.

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Gamero-Sandemetrio, E., Gómez-Pastor, R. & Matallana, E. Zymogram profiling of superoxide dismutase and catalase activities allows Saccharomyces and non-Saccharomyces species differentiation and correlates to their fermentation performance. Appl Microbiol Biotechnol 97, 4563–4576 (2013). https://doi.org/10.1007/s00253-012-4672-1

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  • DOI: https://doi.org/10.1007/s00253-012-4672-1

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