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A novel actinomycete derived from wheat heads degrades deoxynivalenol in the grain of wheat and barley affected by Fusarium head blight

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Abstract

Deoxynivalenol (DON) is a hazardous and globally prevalent mycotoxin in cereals. It commonly accumulates in the grain of wheat, barley and other small grain cereals affected by Fusarium head blight (caused by several Fusarium species). The concept of reducing DON in naturally contaminated grain of wheat or barley using a DON-degrading bacterium is promising but has not been accomplished. In this study, we isolated a novel DON-utilising actinomycete, Marmoricola sp. strain MIM116, from wheat heads through a novel isolation procedure including an in situ plant enrichment step. Strain MIM116 had background degradation activity, and the activity was enhanced twofold by the consumption of DON. Among Tween 20, Triton X-100 and Tween 80, we selected Tween 80 as a spreading agent of strain MIM116 because it promoted DON degradation and the growth of strain MIM116 in the presence of DON. The inoculation of MIM116 cell suspension plus 0.01% Tween 80 into 1,000 harvested kernels of wheat and barley resulted in a DON decrease from approximately 3 mg kg−1 to less than 1 mg kg−1 of dry kernels, even when cells had only basal levels of DON-degrading activity. To the best of our knowledge, this is the first report that describes (1) the isolation of a DON-degrading bacterium from wheat heads, (2) the effects of surfactants on the biodegradation of DON and (3) the decrease of DON levels in naturally contaminated wheat and barley grain using a DON-degrading bacterium.

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Acknowledgements

We thank T. Nakajima, K. Hirayae, A. Miyasaka, F. Suzuki and M. Yoshida, the National Agricultural Research Center for Kyushu Okinawa Region, Koshi, Kumamoto, Japan, for supplying the wheat and barley grain with helpful advice. We thank T. Yoshioka and E. Aoki, the National Institute of Crop Science, Tsukuba, Ibaraki, Japan, for lending us equipment. We also thank our colleagues in the National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan, for helpful discussions. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Research project for ensuring food safety from farm to table MT-3209).

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  1. National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan

    Michihiro Ito, Ikuo Sato, Motoo Koitabashi, Shigenobu Yoshida, Machiko Imai & Seiya Tsushima

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  1. Michihiro Ito
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  2. Ikuo Sato
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  3. Motoo Koitabashi
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  4. Shigenobu Yoshida
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  5. Machiko Imai
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  6. Seiya Tsushima
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Correspondence to Seiya Tsushima.

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Ito, M., Sato, I., Koitabashi, M. et al. A novel actinomycete derived from wheat heads degrades deoxynivalenol in the grain of wheat and barley affected by Fusarium head blight. Appl Microbiol Biotechnol 96, 1059–1070 (2012). https://doi.org/10.1007/s00253-012-3922-6

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  • DOI: https://doi.org/10.1007/s00253-012-3922-6

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