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Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst

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Abstract

We developed a novel enzymatic glutathione (GSH) production system using Saccharomyces cerevisiae as a whole-cell biocatalyst, and improved its GSH productivity by metabolic engineering. We demonstrated that the metabolic engineering of GSH pathway and ATP regeneration can significantly improve GSH productivity by up to 1.7-fold higher compared with the parental strain, respectively. Furthermore, the combination of both improvements in GSH pathway and ATP regeneration is more effective (2.6-fold) than either improvement individually for GSH enzymatic production using yeast. The improved whole-cell biocatalyst indicates its great potential for applications to other kinds of ATP-dependent bioproduction.

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Acknowledgments

We are grateful to Dr. J. Ishii (Organization of Advanced Science and Technology, Kobe University) for providing us with pGK402 and pGK405 plasmids. We thank Dr. Takashi Kondo, Dr. Naoko Okai, and Dr. Kazunori Nakashima (Organization of Advanced Science and Technology, Kobe University) for their helpful discussion. This study was supported by the Special Coordination Funds for Promoting Science and Technology, Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe), MEXT, Japan. Hara KY was supported by Grant-in-Aid for Young Scientists (B) (18769004, 22760608).

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Authors and Affiliations

  1. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1–1 Rokkodaicho, Nada-ku, Kobe, 657–8501, Japan

    Hideyo Yoshida, Kentaro Kiriyama, Chiaki Ogino, Hideki Fukuda & Akihiko Kondo

  2. Organization of Advanced Science and Technology, Kobe University, 1–1 Rokkodaicho, Nada-ku, Kobe, 657–8501, Japan

    Kiyotaka Y. Hara, Hideki Nakayama, Fumiyoshi Okazaki & Fumio Matsuda

Authors
  1. Hideyo Yoshida
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  2. Kiyotaka Y. Hara
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  3. Kentaro Kiriyama
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  4. Hideki Nakayama
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  5. Fumiyoshi Okazaki
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  6. Fumio Matsuda
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  7. Chiaki Ogino
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  8. Hideki Fukuda
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  9. Akihiko Kondo
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Corresponding author

Correspondence to Akihiko Kondo.

Additional information

Hideyo Yoshida and Kiyotaka Y. Hara contibuted equally in this work.

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Yoshida, H., Hara, K.Y., Kiriyama, K. et al. Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst. Appl Microbiol Biotechnol 91, 1001–1006 (2011). https://doi.org/10.1007/s00253-011-3196-4

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  • DOI: https://doi.org/10.1007/s00253-011-3196-4

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